FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_D5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_D5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6008
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC210.34953395472703064No Hit
GGTTAAAGGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAG200.3328894806924101No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT170.2829560585885486No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA170.2829560585885486No Hit
ACTTTACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAA170.2829560585885486No Hit
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA160.2663115845539281No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT150.2496671105193076No Hit
GTGTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAG150.2496671105193076No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG150.2496671105193076No Hit
GAATTACTTTACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGC140.2330226364846871No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC140.2330226364846871No Hit
CAATAATGGTCAACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACA130.21637816245006655No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA130.21637816245006655No Hit
ACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAAGGGGT120.1997336884154461No Hit
CTTTTTGACTGTTTACTCATTTGCCAATAAAACAGTTCACATTTATTGAG120.1997336884154461No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC120.1997336884154461No Hit
GTACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCAT110.18308921438082557No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA100.16644474034620504No Hit
ATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGCCTGTG100.16644474034620504No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA100.16644474034620504No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT100.16644474034620504No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG100.16644474034620504No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG90.14980026631158455No Hit
CTCTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATA90.14980026631158455No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC90.14980026631158455No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA90.14980026631158455No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT90.14980026631158455No Hit
GGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTA80.13315579227696406No Hit
GCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGC80.13315579227696406No Hit
TATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTAACTAACCCCT80.13315579227696406No Hit
GTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCTAATTA80.13315579227696406No Hit
GACATGAGGTCTTAGCCAAGTCACTTCACCACTTTTTGACTGTTTACTCA80.13315579227696406No Hit
GTATTAGACTAATACCTTAGAAATTATGAAAAGTGGGACACTTGGGTGGC80.13315579227696406No Hit
AATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGAACA80.13315579227696406No Hit
GCTGTGGAATGTGTAGCAAGTGAATTAACTAACCCCTTTTAGCTTTAGTA80.13315579227696406No Hit
ATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCACAG80.13315579227696406No Hit
GTGAATTAACTAACCCCTTTTAGCTTTAGTAGGCTCATCTGTGAAATGGA80.13315579227696406No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA70.11651131824234355No Hit
GAATTAACTAACCCCTTTTAGCTTTAGTAGGCTCATCTGTGAAATGGAGA70.11651131824234355No Hit
TGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAGGTAATCGTCAA70.11651131824234355No Hit
TTATATAACAATGCATGGCAAATTTCAAAGGACTGAAATCACGCACAGAG70.11651131824234355No Hit
AACTAACCCCTTTTAGCTTTAGTAGGCTCATCTGTGAAATGGAGATGATG70.11651131824234355No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG70.11651131824234355No Hit
GTTTACTCATTTGCCAATAAAACAGTTCACATTTATTGAGTACACTATAG70.11651131824234355No Hit
GTGTAGCAAGTGAATTAACTAACCCCTTTTAGCTTTAGTAGGCTCATCTG70.11651131824234355No Hit
AGTGAATACTACTTTTGACATAAACCATACGATGATTTATAGGTTTCTTT70.11651131824234355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)