FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5381
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA520.9663631295298271No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT460.8548596915071548No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT200.3716781267422412No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA150.27875859505668094No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC140.2601746887195689No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG130.24159078238245676No Hit
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC120.22300687604534472No Hit
TGTTTGAAATGTGCAATATTGGCAATCAAGACTTACCTCAGCATTTGCTG110.20442296970823265No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT100.1858390633711206No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG100.1858390633711206No Hit
TATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTAACTAACCCCT90.16725515703400856No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA90.16725515703400856No Hit
GATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAA90.16725515703400856No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC90.16725515703400856No Hit
GTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTT90.16725515703400856No Hit
GTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTTTGGTTG80.1486712506968965No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA80.1486712506968965No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC80.1486712506968965No Hit
CTCATATGCATTTGACAAAAGAACTACTGATGGAGTGTTATTATGTCTTA80.1486712506968965No Hit
GTAGGAGACAGAGTGGCATTTTGTACAATATTAAATTCAGGTTCTCAATA80.1486712506968965No Hit
GTCTTAGCCAAGTCACTTCACCACTTTTTGACTGTTTACTCATTTGCCAA80.1486712506968965No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG80.1486712506968965No Hit
GTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAG70.13008734435978445No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA70.13008734435978445No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC70.13008734435978445No Hit
ATATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGA70.13008734435978445No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT70.13008734435978445No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT70.13008734435978445No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC70.13008734435978445No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT70.13008734435978445No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT70.13008734435978445No Hit
ACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGACT60.11150343802267236No Hit
ACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAAGGGGT60.11150343802267236No Hit
TACATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTT60.11150343802267236No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA60.11150343802267236No Hit
CACAGGTTAAGAATCCAGAAAATAATATGGCAGAGTTTTTCAAGAGGAAA60.11150343802267236No Hit
GACTAATACCTTAGAAATTATGAAAAGTGGGACACTTGGGTGGCTCAGTC60.11150343802267236No Hit
CTTTTTGACTGTTTACTCATTTGCCAATAAAACAGTTCACATTTATTGAG60.11150343802267236No Hit
GTATTCTATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCT60.11150343802267236No Hit
GAGCCAAAGGCAAATGCTTAATGATCCTCTATTGGTGCTTCTTACATTCA60.11150343802267236No Hit
TTATATAACAATGCATGGCAAATTTCAAAGGACTGAAATCACGCACAGAG60.11150343802267236No Hit
GGATAGGACAGGGTTGGTTATTGACTGAAACTGGGGATGAGGTAATGCTC60.11150343802267236No Hit
GTCTTAAGAATATGCTGTTAAGAGAACAAGATAAAGGGTGAGTGGGAGAG60.11150343802267236No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG60.11150343802267236No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT60.11150343802267236No Hit
ATATAACAATGCATGGCAAATTTCAAAGGACTGAAATCACGCACAGAGAC60.11150343802267236No Hit
CATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTTCG60.11150343802267236No Hit
TCTCTGGCCTTTTCTCATATGCATTTGACAAAAGAACTACTGATGGAGTG60.11150343802267236No Hit
CTTTTAGAGACAGATAATTAGAGCATTGGTGTCTAGCCCTTTTTTTCAAA60.11150343802267236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)