FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4130
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAAGGGGT80.1937046004842615No Hit
ACTTTACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAA80.1937046004842615No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC80.1937046004842615No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG80.1937046004842615No Hit
GGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTA70.1694915254237288No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT70.1694915254237288No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG70.1694915254237288No Hit
TACATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTT70.1694915254237288No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT70.1694915254237288No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT70.1694915254237288No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA60.14527845036319612No Hit
GTGAAATGGAGATGATGTATGTAAAGTAATTCCATGCTTGGCATCTATAG60.14527845036319612No Hit
GTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGG60.14527845036319612No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC60.14527845036319612No Hit
CTATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGCCTG60.14527845036319612No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG60.14527845036319612No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG60.14527845036319612No Hit
GTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTTTGGTTG50.12106537530266344No Hit
GAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAAATCAGC50.12106537530266344No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC50.12106537530266344No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG50.12106537530266344No Hit
ACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAAT50.12106537530266344No Hit
GTAGCTAGGTAATCGTCAAGGTTTCCCTTTAGCCTAGGGGTTAAAGGTAT50.12106537530266344No Hit
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC50.12106537530266344No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA50.12106537530266344No Hit
CTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTCCAGAAGCCCCAG50.12106537530266344No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG50.12106537530266344No Hit
TGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTGG50.12106537530266344No Hit
GTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAATTAATT50.12106537530266344No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT50.12106537530266344No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT50.12106537530266344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)