FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_D2_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_D2_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAG70.6140350877192983No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA60.5263157894736842No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC50.43859649122807015No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC50.43859649122807015No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA40.3508771929824561No Hit
GTTGGGGTCCTCATCGGAGGACTTGAATGGAATGGTAACACGGTTCGAGT40.3508771929824561No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG40.3508771929824561No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT40.3508771929824561No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT40.3508771929824561No Hit
GTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTGTCTCTTATACA30.2631578947368421No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACCTGTCTC30.2631578947368421No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT30.2631578947368421No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCCT30.2631578947368421No Hit
CAGTTCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTC30.2631578947368421No Hit
CTCACAGACAGATGGCTACTACCACCAATCCACTAATCAGGCATGAAAAC30.2631578947368421No Hit
GCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGCTG30.2631578947368421No Hit
GCATGAGGACATGCTGCCGTTACACCTTTGTTCGAGTCATGATTGGGCCA30.2631578947368421No Hit
GCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGCTGTCTC30.2631578947368421No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT30.2631578947368421No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT30.2631578947368421No Hit
CACTAAGACCACTGTGGACCATATGGCCACTGTCTCTTATACACATCTGA30.2631578947368421No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT30.2631578947368421No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT30.2631578947368421No Hit
CACCATAGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAG30.2631578947368421No Hit
CTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCCTGTCTCTTA20.17543859649122806No Hit
CTTCAGCTCAGGTCATGATCCCAGGGTCCTGGGATGGAGCCCTGCATTGA20.17543859649122806No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG20.17543859649122806No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC20.17543859649122806No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC20.17543859649122806No Hit
GACCTGAGCTGAAGGCAGATGTTTAACTGACTGAGCCACCCAAGTGCTGT20.17543859649122806No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG20.17543859649122806No Hit
GTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTC20.17543859649122806No Hit
CCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAG20.17543859649122806No Hit
GATCTTGAAAGAGGAATCCAGCGAGACACTTAGAATGACAATTGCATCTG20.17543859649122806No Hit
GCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCCT20.17543859649122806No Hit
AAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTG20.17543859649122806No Hit
GCAATGAGATACCCAATTACAGCAGACAGAGGATTTCTCATTTTGGGCAA20.17543859649122806No Hit
ATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCATAA20.17543859649122806No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCTGTCTCTTATAC20.17543859649122806No Hit
GTGTTGGAGTCCATTATGTTTTTGTCACCCTGCTTTTGCTCCTGTCTCTT20.17543859649122806No Hit
ATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCTCTAT20.17543859649122806No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20.17543859649122806No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG20.17543859649122806No Hit
GCTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGA20.17543859649122806No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA20.17543859649122806No Hit
AAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAA20.17543859649122806No Hit
CTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCTGTCTCTTATACAC20.17543859649122806No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGA20.17543859649122806No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA20.17543859649122806No Hit
TAGTTGGAGAAATTTCACCATTACCTTCTCTTCCTGTCTCTTATACACAT20.17543859649122806No Hit
GGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCT20.17543859649122806No Hit
ACTTCAGATAGGTAAGTCTCCATGAATGATGTCCAAGTCAGTAGTTCAAA20.17543859649122806No Hit
TTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCT20.17543859649122806No Hit
GAGAAGGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAACTGT20.17543859649122806No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT20.17543859649122806No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA20.17543859649122806No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA20.17543859649122806No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT20.17543859649122806No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT20.17543859649122806No Hit
GTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGT20.17543859649122806No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC20.17543859649122806No Hit
AAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAG20.17543859649122806No Hit
GTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGAT20.17543859649122806No Hit
CAGGAAGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCT20.17543859649122806No Hit
ATGCTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG20.17543859649122806No Hit
CACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAATTC20.17543859649122806No Hit
CCCCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT20.17543859649122806No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATCTGTCTCTT20.17543859649122806No Hit
CTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACA20.17543859649122806No Hit
GTACATGCAATGAGAACTATTGGGACTCATCCTAGCTCCAGTGCTGGTCT20.17543859649122806No Hit
GTGCGGGGTTCTTCTCTTGCCTTCCTGATGCTGTCTCTTATACACATCTG20.17543859649122806No Hit
GCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGG20.17543859649122806No Hit
GAGCCGATCAAGGAATGGGGCATCACCCAATCCATTGTCTGCAAATCGCT20.17543859649122806No Hit
CACAGACAGATGGCTACTACCACCAATCCACTAATCAGGCATGAAAACAG20.17543859649122806No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG20.17543859649122806No Hit
CAATGATATTTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCATCTG20.17543859649122806No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT20.17543859649122806No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG20.17543859649122806No Hit
AAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC20.17543859649122806No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA20.17543859649122806No Hit
CTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTGTCTCTTA20.17543859649122806No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA20.17543859649122806No Hit
CACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATT20.17543859649122806No Hit
AGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGCTGTCT20.17543859649122806No Hit
CAGTCCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGG20.17543859649122806No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA20.17543859649122806No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCCTG20.17543859649122806No Hit
AGTTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGC20.17543859649122806No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG20.17543859649122806No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG20.17543859649122806No Hit
CCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACCTGTCTC20.17543859649122806No Hit
GTGTTGGAGTCCATTATGTTTTTGTCACCCTGCTTTCGCTCCTGTCTCTT20.17543859649122806No Hit
GTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAG20.17543859649122806No Hit
GTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAATGTGGTGCTTAT20.17543859649122806No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAG20.17543859649122806No Hit
ATCCAGCGAGACACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCT20.17543859649122806No Hit
GGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATC20.17543859649122806No Hit
GTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTG20.17543859649122806No Hit
GCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAA20.17543859649122806No Hit
GTGTTGGAGTCCATTATGTTTTTGTCACCCTGCTTTTGCCTGTCTCTTAT20.17543859649122806No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGCTG20.17543859649122806No Hit
GACAACTAATTGATGGCCATCCGAATTCTTTTGGCTGTCTCTTATACACA20.17543859649122806No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT20.17543859649122806No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCTGTCTCTTATA20.17543859649122806No Hit
GGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGAT20.17543859649122806No Hit
CTTCAATAGTGCATTCGGGTCACCAGATTCTACAATTATTGATTCACCCC20.17543859649122806No Hit
TTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGAT20.17543859649122806No Hit
GAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCTCAGTGAAAGC20.17543859649122806No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA20.17543859649122806No Hit
TTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTCTGTCTCTTAT20.17543859649122806No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA20.17543859649122806No Hit
GCTTTTGGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCA20.17543859649122806No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG20.17543859649122806No Hit
TTCCTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT20.17543859649122806No Hit
CCCTAAGAACATGCAGGAAAAGAGAGTGGCATCTTCAACAAATGAATAAT20.17543859649122806No Hit
ATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCC20.17543859649122806No Hit
GGTGAAATTTCTCCAACTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAG20.17543859649122806No Hit
TGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGCTGTCTCTTAT20.17543859649122806No Hit
CTTCAGTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTT20.17543859649122806No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCTGTCTCTTATAC20.17543859649122806No Hit
GGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTGTCT20.17543859649122806No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACCTGTCTCTTATAC20.17543859649122806No Hit
CAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCG20.17543859649122806No Hit
AGCGAAAGCAGGACAATTTGAATTTCCAAACTCAGTAAGAGGGAAACAAG20.17543859649122806No Hit
GAACAGGCAGCGGAGGCCATGGAGGTTGCTCTGTCTCTTATACACATCTG20.17543859649122806No Hit
CCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTC20.17543859649122806No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG20.17543859649122806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)