FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_C5_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_C5_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5527
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC180.325673964175864No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT130.23520897412701286No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC110.1990229781074724No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC100.18092998009770217No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG100.18092998009770217No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA100.18092998009770217No Hit
GTCTTGAATAAGTGGAAGGCTTCTGCCAGATTATCTAACGTCAGTTTGTT90.162836982087932No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC80.14474398407816175No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG80.14474398407816175No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA80.14474398407816175No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC80.14474398407816175No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT80.14474398407816175No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA80.14474398407816175No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG80.14474398407816175No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG70.12665098606839154No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA70.12665098606839154No Hit
GTATAGTGACTCAGCAGTTACTCAGAGCTTCTCACAGAAAGTGTACTCCT70.12665098606839154No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA70.12665098606839154No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT70.12665098606839154No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG70.12665098606839154No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG60.1085579880586213No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC60.1085579880586213No Hit
CCTAGAGAGTGTGTGAGGTGAGAAGAGCAGAGAAGGATGGAATCTGAGAG60.1085579880586213No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA60.1085579880586213No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA60.1085579880586213No Hit
GGCCTGCACTGAGGAAGAAGGCAGAAGACGGAGAACTGTCAGGAAACAAA60.1085579880586213No Hit
CTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT60.1085579880586213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)