FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_C5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_C5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT90.28994845360824745No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATATT90.28994845360824745No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA80.25773195876288657No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT80.25773195876288657No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT70.22551546391752575No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC70.22551546391752575No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATATTCGAGA70.22551546391752575No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA70.22551546391752575No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT60.19329896907216496No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT60.19329896907216496No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT60.19329896907216496No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA60.19329896907216496No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT60.19329896907216496No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC60.19329896907216496No Hit
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA60.19329896907216496No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC60.19329896907216496No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT60.19329896907216496No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA50.1610824742268041No Hit
GAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCTCAGTG50.1610824742268041No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC50.1610824742268041No Hit
AAGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCT50.1610824742268041No Hit
CTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCT50.1610824742268041No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA50.1610824742268041No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT50.1610824742268041No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA50.1610824742268041No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT50.1610824742268041No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATATCCTGTTCCTGTTCC50.1610824742268041No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC50.1610824742268041No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG40.12886597938144329No Hit
CTTCCAGGACATACTTATGAGGATGTTAAAAATGCAGTTGGGGTCCTCAT40.12886597938144329No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA40.12886597938144329No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT40.12886597938144329No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA40.12886597938144329No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC40.12886597938144329No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC40.12886597938144329No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT40.12886597938144329No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATATCCTGTTCCTGTT40.12886597938144329No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT40.12886597938144329No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC40.12886597938144329No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT40.12886597938144329No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG40.12886597938144329No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT40.12886597938144329No Hit
GGACATACTTATGAGGATGTTAAAAATGCAGTTGGGGTCCTCATCGGAGG40.12886597938144329No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC40.12886597938144329No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT40.12886597938144329No Hit
CAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGGTGTTGCCTCTTCCT40.12886597938144329No Hit
GGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGAC40.12886597938144329No Hit
GTGGAATGTGTAGCAAGTGAATTAACTAACCCCTTTTAGCTTTAGTAGGC40.12886597938144329No Hit
CTTATGAGGATGTTAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA40.12886597938144329No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT40.12886597938144329No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG40.12886597938144329No Hit
ACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAG40.12886597938144329No Hit
CTTTTGAGCTTCTTGTATAGTTTAACTGCTCTATCCATGTTGTTCGGGTC40.12886597938144329No Hit
CCTTACAACTACTGCTTGAAGTAGAACAAGAGATAAGAGCTTTCTCGTTT40.12886597938144329No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT40.12886597938144329No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)