FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_C3_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_C3_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4230
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG80.18912529550827423No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA70.16548463356973994No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA70.16548463356973994No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG70.16548463356973994No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA60.14184397163120568No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC60.14184397163120568No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG50.1182033096926714No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC50.1182033096926714No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA50.1182033096926714No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT50.1182033096926714No Hit
GTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGAGCACAGCATAA50.1182033096926714No Hit
GTTGTTAAGTGTCTGCCTTCAGCTCAGGTCATGATTCCAAAGTCCTGGGA50.1182033096926714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)