FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_B5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_B5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCATGAGTGTCTCCAAGATGCATATCTTTGTTTTTAATTCCCTGTC30.2926829268292683No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG30.2926829268292683No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA20.1951219512195122No Hit
ACAGGGAAGAGAGGAGTTAGAAAGAAGACAAAGCCAGGGTGCCTCAGGGG20.1951219512195122No Hit
ACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAG20.1951219512195122No Hit
CTTAATGACTGAGCCACCCAGGCACCCCTCAGTACATCTAAATCCTGTCT20.1951219512195122No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC20.1951219512195122No Hit
GAATACAACATTCCACGCTAACATGATTCAAATGATGTTTCCAGGATGCC20.1951219512195122No Hit
GGGCACGGATTGCATGGACCACTGGGTGTGGTGCAAAAATCTGTCTCTTA20.1951219512195122No Hit
TAATCAAAAAGTACACATCAGGGAGGCCTGTCTCTTATACACATCTGACG20.1951219512195122No Hit
TGCTTGAACTCTCTGGCCTTTTCTCATATGCATTTGACAAAAGAACTACT20.1951219512195122No Hit
CATCAGGAAGGCAAAAGGAGAGAACGCTAATGCTGTCTCTTATACACATC20.1951219512195122No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAGAAGCAGGCTTCTCACGAACA20.1951219512195122No Hit
CTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTC20.1951219512195122No Hit
CTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTGTCTCTT20.1951219512195122No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC20.1951219512195122No Hit
GAGCAAAAGCAGGGGTAGTGGCAGACAAAGGGAGCAGTAGGCTCCTCTGC20.1951219512195122No Hit
CTTTCTGTGAGAAGCTCTGAGTAACTGCTGAGTCACTATACCTGTCTCTT20.1951219512195122No Hit
GGTGATCATCACGCCCAAGTCAAAAAGTCAGTGTACTTCCCAAATTAGAG20.1951219512195122No Hit
GGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGCTGTCTCTTATACACA20.1951219512195122No Hit
GGATCGACATCCATTCAAATGGTTTGCCTGCTTTCGCTCTGTCTCTTATA20.1951219512195122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)