FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_B4_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_B4_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATAT40.2706359945872801No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA40.2706359945872801No Hit
GCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTT40.2706359945872801No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT40.2706359945872801No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT30.20297699594046006No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT30.20297699594046006No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30.20297699594046006No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC30.20297699594046006No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG30.20297699594046006No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.20297699594046006No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC30.20297699594046006No Hit
ACGGAAGGAGTGCCTGAGTCCATGAGGGAAGAATATCAACAGGAACAGCA30.20297699594046006No Hit
GTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGG30.20297699594046006No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG30.20297699594046006No Hit
AGATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCATTTC30.20297699594046006No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT30.20297699594046006No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT30.20297699594046006No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT30.20297699594046006No Hit
GGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTCTGTCTCTTATA20.13531799729364005No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGCTGTCTCTTATACACA20.13531799729364005No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTC20.13531799729364005No Hit
ACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTT20.13531799729364005No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT20.13531799729364005No Hit
CTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGT20.13531799729364005No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCTGT20.13531799729364005No Hit
CAGCTGGCTGGGCCCGCAGCGGAGGCCGACCCGCTGGGCCGCTTTACGTG20.13531799729364005No Hit
ATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCATAA20.13531799729364005No Hit
CTCCAGAAATTTCGGTCATTGATTCCACGTTTGATCATTCTGATTAACTC20.13531799729364005No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT20.13531799729364005No Hit
TATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTGACA20.13531799729364005No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG20.13531799729364005No Hit
TTCACATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCAT20.13531799729364005No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG20.13531799729364005No Hit
GCTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGG20.13531799729364005No Hit
TGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTCTAAGT20.13531799729364005No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA20.13531799729364005No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT20.13531799729364005No Hit
CGTGCCTTCCTTTGACATGAGTAATGAAGGGTCTTATTTCCTGTCTCTTA20.13531799729364005No Hit
TACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCA20.13531799729364005No Hit
GGATTCCTCTTTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGG20.13531799729364005No Hit
GTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTGGGAAG20.13531799729364005No Hit
TCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGG20.13531799729364005No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT20.13531799729364005No Hit
GGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA20.13531799729364005No Hit
GTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCC20.13531799729364005No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC20.13531799729364005No Hit
GCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGC20.13531799729364005No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20.13531799729364005No Hit
CCACAAGAGGGGTCCAGATTGCTTCAAATGAGAATGTGGCTGTCTCTTAT20.13531799729364005No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA20.13531799729364005No Hit
GCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATG20.13531799729364005No Hit
ATTCTGGATTAGTCGTCCCTCATAATCACTGAGTTTGAGTTCAGTGCACA20.13531799729364005No Hit
GAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGG20.13531799729364005No Hit
GGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGAGAATAGTTTC20.13531799729364005No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAGGATCCTGTCTCTTATAC20.13531799729364005No Hit
ATTACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCT20.13531799729364005No Hit
TCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATC20.13531799729364005No Hit
GCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACT20.13531799729364005No Hit
GTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTACATCTGTCTCTTAT20.13531799729364005No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCTGTC20.13531799729364005No Hit
GTGGATGATGGCAATGAGATACCCAATTGAGAGGATTTCTCATTTTGGGC20.13531799729364005No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA20.13531799729364005No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20.13531799729364005No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGGGA20.13531799729364005No Hit
TAGTGGCACAGTACTAGAAATGCTGAAGCAAAAGTCGTGACTACTCCTTA20.13531799729364005No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT20.13531799729364005No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.13531799729364005No Hit
CATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCT20.13531799729364005No Hit
AAGCGAATCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCAT20.13531799729364005No Hit
GCAATCTGTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGT20.13531799729364005No Hit
GATCCATTCCACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGA20.13531799729364005No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT20.13531799729364005No Hit
TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGA20.13531799729364005No Hit
CTCCATGGCCTCCGCTGCCTGTTCACTCGATCCAGCCATCTGTTCCATAG20.13531799729364005No Hit
AATCGACCGTGGGTGTCTTTCAACCAGAATCTGGAATATCAGATAGGATA20.13531799729364005No Hit
CATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATT20.13531799729364005No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG20.13531799729364005No Hit
GGTTGTGATCCCATGGTCCTGGGATAGAGCCCTGCATCAGGCTCCCTGCT20.13531799729364005No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA20.13531799729364005No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA20.13531799729364005No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT20.13531799729364005No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG20.13531799729364005No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC20.13531799729364005No Hit
CTCAGTGGGAAGCCTGCTTCTCCCTCTCCCACTCGCCCTGCTGTCTCTTA20.13531799729364005No Hit
CTTCAGTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTT20.13531799729364005No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTCTGTCTCTTATACACATCTG20.13531799729364005No Hit
ATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTT20.13531799729364005No Hit
AGAGAATAATGGACATGATTCCAGAGAGGAATGAACCTGTCTCTTATACA20.13531799729364005No Hit
CTCTATTGTTATGCTATTCTGGATTAGTCGTCCCTCATAATCACTGAGTT20.13531799729364005No Hit
GTATTTATCGTCGCTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAA20.13531799729364005No Hit
AATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTATACAAT20.13531799729364005No Hit
TCTTCTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGG20.13531799729364005No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC20.13531799729364005No Hit
CTGGGCACGGTGAGCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGG20.13531799729364005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)