FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_B2_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_B2_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA50.441696113074205No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA40.35335689045936397No Hit
CAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTG40.35335689045936397No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA30.26501766784452296No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG30.26501766784452296No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT30.26501766784452296No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG30.26501766784452296No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC30.26501766784452296No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC30.26501766784452296No Hit
GCTTATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATACT20.17667844522968199No Hit
ACCCAATCCATTGTCTGCAAATCGCTTGCGGATATGCCAAAGGAAACAGT20.17667844522968199No Hit
ATCCAGCCATCTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATT20.17667844522968199No Hit
GTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGTTTCCCAAC20.17667844522968199No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG20.17667844522968199No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT20.17667844522968199No Hit
GTCTATATCTGCCCCCCTAAAATGCCCAGGGAGTGAGTGCCCGGGCACAG20.17667844522968199No Hit
GCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAG20.17667844522968199No Hit
ATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAG20.17667844522968199No Hit
TCATTGTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTT20.17667844522968199No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCCTGTCTCTTATACACATCTG20.17667844522968199No Hit
ATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGGAGGT20.17667844522968199No Hit
GAGCAAAAGCAGGGGGAGCTGTGGAGGAAGAGGGAGAAGCAGACCTGTCT20.17667844522968199No Hit
GTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAA20.17667844522968199No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCCTGTC20.17667844522968199No Hit
GTGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTCAACC20.17667844522968199No Hit
GACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGGGCACTGTCTCTTA20.17667844522968199No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT20.17667844522968199No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA20.17667844522968199No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG20.17667844522968199No Hit
GTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAG20.17667844522968199No Hit
ACATCGGGCTCTCTGCTCAGAGGGGAGCCTGCTTCCTTTTCCTGTCTCTT20.17667844522968199No Hit
TGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGCCTG20.17667844522968199No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20.17667844522968199No Hit
AGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTCTGTCTCTTATA20.17667844522968199No Hit
CACCGGGGGGTCTGCTTGTCCCTCTCCCTCTGCCCCTCCACCTGCTTTTG20.17667844522968199No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC20.17667844522968199No Hit
TAGAGACCTTTGGTTTCTTTTTCTCTAAGGCCTGTCTCTTATACACATCT20.17667844522968199No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT20.17667844522968199No Hit
AACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAGCGCGA20.17667844522968199No Hit
CAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTT20.17667844522968199No Hit
TGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGGAGGTTGCTAATCAG20.17667844522968199No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG20.17667844522968199No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20.17667844522968199No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT20.17667844522968199No Hit
CTGTATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGT20.17667844522968199No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG20.17667844522968199No Hit
CAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGAT20.17667844522968199No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)