FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_A9_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_A9_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238143
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA36691.5406709414091533No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC23180.9733647430325476No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC22920.962446933145211No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA22800.9574079439664404No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC22240.9338926611321768No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA21750.9133167886521965No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA20280.8515891712122547No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC16510.6932809278458741No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG15090.6336528892304204No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA13380.5618472934329374No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG11250.4724052355097567No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG10780.4526691945595714No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG10460.43923189008284935No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA9860.41403694418899567No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA9750.40941787077512254No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG9750.40941787077512254No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA9430.39598056629840056No Hit
ATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAG8230.34559067451069314No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT7920.33257328579886875No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA7880.33089362273927847No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT7700.3233351389711224No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC7230.303599098020937No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCTCCTGTCT6880.28890204624952237No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT6670.2800838151866736No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG6470.2716854998887223No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG6440.2704257525940296No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGA6060.2544689535279223No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCT6010.2523693747034345No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTG5850.24565072246507352No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA5810.24397105940548325No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA5800.24355114364058567No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT5680.23851215446181495No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC5540.2326333337532491No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC5540.2326333337532491No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA5470.2296939233989662No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT5410.2271744288095808No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG5160.21667653468714176No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA5110.21457695586265396No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT5000.20995788244878077No Hit
CAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG4990.2095379666838832No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA4980.20911805091898567No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA4980.20911805091898567No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC4930.20701847209449784No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG4900.20575872479980514No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT4790.20113965138593196No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC4770.20029981985613685No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA4770.20029981985613685No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG4770.20029981985613685No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA4710.19778032526675146No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC4660.19568074644226366No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC4570.19190150455818564No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG4480.1881222626741076No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT4390.1843430207900295No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC4380.18392310502513196No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC4270.17930403161125877No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT4260.1788841158463612No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC4190.1759447054920783No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA4140.1738451266675905No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC4040.16964596901861487No Hit
ACATTAGATCTCTCAGTTCTTTTATTCTCTCCATATTGAATATAATTGAC4030.1692260532537173No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA4030.1692260532537173No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT4010.16838622172392217No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCCTGTCTCT3930.16502689560474168No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA3880.16292731678025388No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT3820.16040782219086852No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA3810.15998790642597097No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT3780.15872815913127827No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA3700.15536883301209778No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC3700.15536883301209778No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCA3540.1486501807737368No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA3440.14445102312476119No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC3420.14361119159496605No Hit
GCCATATCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGG3310.13899211818109286No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA3310.13899211818109286No Hit
ACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATC3250.1364726235917075No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCTCT3170.133113297472527No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA3150.13227346594273187No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA3090.12975397135334654No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTCTCTCCATA3040.1276543925288587No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC2990.1255548137043709No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT2960.12429506640967822No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC2940.12345523487988311No Hit
ATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGA2930.12303531911498553No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA2920.12261540335008797No Hit
GGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCA2830.11883616146600992No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG2810.1179963299362148No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC2810.1179963299362148No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT2800.11757641417131724No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCT2800.11757641417131724No Hit
ATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACA2790.11715649840641969No Hit
ATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTC2780.1167365826415221No Hit
GAATACTGGAGAATTGCACCTTTCGACCTTTTCGAAATAAGTTTTATATA2780.1167365826415221No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA2780.1167365826415221No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA2770.11631666687662455No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC2750.11547683534682941No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT2750.11547683534682941No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG2740.11505691958193186No Hit
GTGCGGGACTGCGACATTAGATCTCTCAGTTCTTTTATTCTCTCCATATT2740.11505691958193186No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA2720.11421708805213675No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT2690.11295734075744406No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT2680.1125374249925465No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT2670.11211750922764893No Hit
TGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGAC2640.11085776193295625No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG2640.11085776193295625No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTGCCATCATCCACTTCATTCTG2610.10959801463826357No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA2580.10833826734357088No Hit
CTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGC2540.10665860428398063No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCTCCAGTATTCA2510.10539885698928794No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG2490.10455902545949283No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA2490.10455902545949283No Hit
GAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGA2450.10287936239990257No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC2430.10203953087010746No Hit
GTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCT2400.10077978357541477No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG2400.10077978357541477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)