FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P3_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG40.35211267605633806No Hit
TCTCTGGTCAGCAGGGAGCCTGCTTCTCCCCTGTCTCTTATACACATCTG30.2640845070422535No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC30.2640845070422535No Hit
GATAAAGGGTGAGTGGGAGAGTAAATAAGTAGAATGAGAAGGAAAACCCA30.2640845070422535No Hit
CCCATTTCCTTTGCTTGAACTCTCTGGCCTTTTCTCATATGCATTTGACA30.2640845070422535No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT30.2640845070422535No Hit
GGCCTGGGGAGGCCCAGGCGCCGGAACCCAGCCACTGCTGAATTCCCAGA30.2640845070422535No Hit
CAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACCGTGTT20.17605633802816903No Hit
CCCAATTGCTCTCGCCACTTTTCATTTCTGCTCTGGAGGTAGTGAAGGCT20.17605633802816903No Hit
TTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGG20.17605633802816903No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG20.17605633802816903No Hit
ATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATATTGT20.17605633802816903No Hit
GACTCCCCGCCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATCA20.17605633802816903No Hit
GGTACATGCAATGAGAACTATTGGGACTCATCCTAGCTCCAGTGCTGGTC20.17605633802816903No Hit
CCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCATAAGTATGTCC20.17605633802816903No Hit
CTCCACGTACTGCCTGTCCCACGTGCACGGTGCATTTCCCTGTCTCCCTG20.17605633802816903No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCCTGTCTCTTATACA20.17605633802816903No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC20.17605633802816903No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA20.17605633802816903No Hit
GACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAA20.17605633802816903No Hit
TGTTGGGAAACAAATCGTGGAATGGATCTTGAAAGAGGAATCCAGCGAGA20.17605633802816903No Hit
GTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAG20.17605633802816903No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATCTGTC20.17605633802816903No Hit
GAGCAAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTGTATTC20.17605633802816903No Hit
GTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGCACTGGAG20.17605633802816903No Hit
AGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCCTGTCTCTTAT20.17605633802816903No Hit
GGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGT20.17605633802816903No Hit
GGCAGAAGACGGAGAACTGTCAGGAAACAAACTGACGTTAGATAATCTGG20.17605633802816903No Hit
ACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGTGAACC20.17605633802816903No Hit
GTCTTGTCTTTAGCCATTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCT20.17605633802816903No Hit
GGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCCTGTCTCTTAT20.17605633802816903No Hit
CAATAATTGGTGTCCTTTGTTAAGCGCTATTATGTGTCCTAGAAACCTAT20.17605633802816903No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC20.17605633802816903No Hit
TCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGTTGTGGCAATG20.17605633802816903No Hit
CGCGCAGAGACTGGAAAGTGTCTTTGCAGGAAAGAACCTGTCTCTTATAC20.17605633802816903No Hit
CTCCCCAAAGTAGTCTTTCCTGATGTCTGACTGCTCCTTTTTCATGGGTA20.17605633802816903No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC20.17605633802816903No Hit
GGCTTGCCCGTTAAAAAACCCCAGAGAAAACAGTTCCTGCTGCATTATTG20.17605633802816903No Hit
CCATGGACACATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCT20.17605633802816903No Hit
CCCAATCCATTGTCTGCAAATCGCTTGCGGATATGCCAAAGGAAACAGTC20.17605633802816903No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.17605633802816903No Hit
CTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAAT20.17605633802816903No Hit
GCGCTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACC20.17605633802816903No Hit
CGGCGGAGCCGATCAAGGAATGGGGCATCACCCAATCCATTGTCTGCAAA20.17605633802816903No Hit
CACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTC20.17605633802816903No Hit
AACTAACCCCTTTTAGCTTTAGTAGGCTCATCTGTGAAATGGAGATGATG20.17605633802816903No Hit
TGGTTAGGATTTGCATCTTTCACTTGCTGAATGTAAGAAGCACCAATAGA20.17605633802816903No Hit
CCCTTTATCTTGTTCTCTTAACAGCATATTCTTAAGACTGACCTATGTTG20.17605633802816903No Hit
GGGGAGCAGCAGAGGGAGAAGGAGAAGTGGGCTCCCCACTGAGCAGGGAG20.17605633802816903No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATT20.17605633802816903No Hit
GATGGAGTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATG20.17605633802816903No Hit
GAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGA20.17605633802816903No Hit
AGACTTACCTCAGCATTTGCTGGGACACAGGAAACCAGCTAATGGCTGAT20.17605633802816903No Hit
GTGTTGTCACTGGATTTTTACATCAGGTGATTATTGTTCTTTCTCCCCAA20.17605633802816903No Hit
ACTTGGAGATCATATGTGAAGTAAAATAAGCTAGTTGTCAAACGACAAGT20.17605633802816903No Hit
GACTGGAGCAGACTGCCCAAGTTCAAATCTCATCCTGTGCTCCTGTCTCT20.17605633802816903No Hit
ACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGAC20.17605633802816903No Hit
TGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAGGTAATCGTCAA20.17605633802816903No Hit
GTCTAAAAGTATTGCTCTTCGACTAACTAGAATTCCCAAGATGGGGAAGG20.17605633802816903No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATCTG20.17605633802816903No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT20.17605633802816903No Hit
GCAGATGGTACATGCAATGAGAACTATTGGGACTCATCCTAGCTCCAGTG20.17605633802816903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)