FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_H5_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_H5_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9479379
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC1024371.0806298598252058No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG857670.9047744583268588No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA819560.8645714028313458No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT787200.8304341455278873No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA740390.7810532736374397No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT662700.6990964281520973No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA421720.4448814632266523No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC419340.4423707502358541No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG414780.43756030853919864No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT405460.4277284408609467No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT392410.41396171626854456No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC376750.397441646757662No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA359580.37932864589547477No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA356460.37603729105039474No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC347360.36643750608557796No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA340900.3596227136819828No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA336840.3553397326976799No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA331040.34922118843439004No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT330580.3487359245790257No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA330440.348588235579567No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA330020.3481451685811908No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC329740.3478497905822734No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT316770.3341674597038477No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC309830.326846305016394No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT309440.32643488566075896No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT304230.3209387450380452No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA304210.3209176466095511No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA302410.3190187880450819No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC296780.3130795804239919No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG292250.30830078637007763No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT285050.30070535211220056No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC279270.2946079062774049No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG277720.29297277806911187No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC273710.2887425431560443No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC268710.28346793603251863No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG263990.27848870690791033No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA262630.27705401377031136No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC259590.27384705263920767No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC257250.27137853650539767No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA255370.269395284226952No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC251560.2653760335988254No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT244530.25795993598314826No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT240000.25318114192923397No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA234390.24726303273663813No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA229570.24217831146955937No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG225840.23824345455540916No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA222230.23443518821222362No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC221060.23320093014531862No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG220600.23271566628995421No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA216130.22800016752152225No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC212000.22364334203749No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT211730.2233585132528196No Hit
ATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTC209120.22060516833433919No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA205730.21702898470458876No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG199690.21065725929936974No Hit
CTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCA198740.20965508394589985No Hit
CAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG195950.20671185317097251No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA195060.2057729731029849No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA195020.2057307762459967No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA192720.2033044569691749No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC192030.20257656118612832No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA191560.2020807481165169No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG190850.20133175390497624No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG190520.20098362983482354No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC189200.1995911335542128No Hit
TGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTC184670.1948123395002985No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA183160.19321940814899374No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA181420.19138384487000676No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA177760.18752283245558596No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA173590.18312381011456552No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA173420.18294447347236564No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA170640.18001179191168534No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA170460.17982190605523843No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA170010.17934719141412112No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG169060.17834501606065123No Hit
GAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGG168740.17800744120474557No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTT167850.177068561136758No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG162960.17190999536994986No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA162020.17091836923072704No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC161400.17026431794740984No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA158190.16687802017410636No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA157170.16580200032090708No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA154910.16341787790107348No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC154440.16292206483146204No Hit
GTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATT154270.1627427281892622No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT154020.1624789978330859No Hit
GTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCT153890.16234185804787424No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC153530.16196208633498038No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG152910.1613080350516632No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG152670.16105485390973395No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT151340.1596518084148761No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCA150680.15895556027457075No Hit
CTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATT150370.15862853463291215No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG150270.15852304249044163No Hit
GGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTC147370.15546377035879672No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT146880.1549468588606912No Hit
CTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT144490.1524255966556459No Hit
ATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTA144410.15234120294166947No Hit
TCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTG144170.15208802179974026No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT142650.15048454123418845No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA142420.15024190930650627No Hit
GAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGA142090.14989378523635355No Hit
CCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCA141710.1494929150949656No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA141310.14907094652508354No Hit
CTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT140150.1478472376724256No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA139040.14667627489100288No Hit
GAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCT138330.14592728067946223No Hit
ATGCTGGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGG136600.14410226661472234No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGA136050.1435220598311345No Hit
CTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGG132400.13967159663096074No Hit
GGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATG132300.13956610448849022No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG130560.1377305412095033No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT130280.13743516321058583No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC128170.135209279004458No Hit
GGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGC127010.13398557015180002No Hit
GTATCACCTCTGGCCGTAACATGGTGGAATAGGAATGGCCCAACAACAAG125280.13216055608706012No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA123640.13043048495054368No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA123440.13021950066560267No Hit
CAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAA123320.13009291009463805No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCA122200.1289113980989683No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC122170.12887975045622715No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG120620.12724462224793417No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA120190.12679100603531096No Hit
TGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGT118450.12495544275632402No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA118440.12494489354207697No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT117490.12394271818860708No Hit
ATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT117470.12392161976011297No Hit
GTGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAG116550.12295109204938424No Hit
ATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTT115070.12138980834082065No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAAC115060.12137925912657357No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA114310.12058806805804473No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG112220.11838328228041099No Hit
GTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCC112200.11836218385191688No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT112170.11833053620917573No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA111370.11748659906941161No Hit
ATATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC111270.1173811069269411No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT109980.11602025828907146No Hit
GATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCT109810.1158409216468716No Hit
GCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTA105510.11130475952063948No Hit
GCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTA104410.11014434595346384No Hit
CGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTCTGGAATC103050.10870965281586484No Hit
TTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTC102060.10766528060540674No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC101440.10701122932208955No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAACTGTCTC101410.10697958167934842No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA101340.10690573717961904No Hit
AATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTA100710.1062411366820548No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTCTGTCTC100300.10580861889792569No Hit
GTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGA99720.10519676447159673No Hit
GGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTC99650.10512291997186736No Hit
GAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAA99340.10479589433020876No Hit
CTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGA98650.10406799854716221No Hit
CTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCC98580.10399415404743284No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACCTGTCTC97550.10290758497998656No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG97360.10270714990929258No Hit
ACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCA96940.10226408291091642No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCTGTCTC96410.1017049745558227No Hit
CTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCA95510.10075554527358807No Hit
CCAGAGGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTC94810.10001710027629446No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)