FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_G8_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_G8_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4455574
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT296300.6650097159198792No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC192350.4317064423124832No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT179050.4018561918172608No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC164110.36832515855420644No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA150410.33757715616439093No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG129670.2910287204297359No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA126700.2843629126123817No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT122820.2756547192348281No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC120090.2695275625542298No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT119290.2677320587650435No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA116240.2608867005687707No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT111730.2507645479572329No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT110420.24782441050244033No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA109070.2447944978581884No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT108770.24412118393724358No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT107170.24053017635887095No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA103920.23323594221530156No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA101870.22863496375551162No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC101220.22717611692679773No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT100570.2257172700980839No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC100050.22455019263511278No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA99870.22414620428254586No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT96340.2162235438127613No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC93070.20888442207446226No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC88700.199076482626032No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG87230.19577724441340216No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA84990.19074983380368052No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT83640.18771992115942862No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA82850.18594686116760714No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG82830.18590197357287747No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC81310.18249051637342348No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT80690.1810990009368041No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC80560.18080723157106132No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC79890.17930349714761779No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC79870.17925860955288814No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT79140.17762021234525563No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG77470.1738720981853292No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC75610.16969755187547103No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT74140.1663983136628412No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT73870.16579233113399083No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG70860.15903674812717733No Hit
CTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTA70760.15881231015352903No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA70590.15843076559832694No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA70240.15764523269055794No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC69510.15600683548292543No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG68340.15338091119124045No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT68130.15290959144657904No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA67810.1521913899309045No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT67770.15210161474144523No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT67280.15100186867056858No Hit
GCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTT65520.1470517603343587No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG65470.14693954134753456No Hit
CATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATT65270.146490665400238No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT64990.1458622390740228No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG63850.1433036461744323No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC62270.13975752619078935No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA62010.1391739874593038No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG61220.1374009274674823No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC60540.13587474924667395No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA59580.13372014469965038No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG58110.13042090648702054No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA57870.12988225535026462No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC57320.12864784649519906No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA57030.127996976371619No Hit
TAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGC56830.12754810042432244No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT56460.12671767992182376No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC55540.12465285056425951No Hit
ATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTC55390.12431619360378707No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG55060.12357554829074772No Hit
CTTCAGTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTT54820.12303689715399183No Hit
AAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAG54640.12263290880142491No Hit
GTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACT54250.1217576007041966No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA53510.12009675969919925No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT53440.11993965311764546No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG52760.1184134748968371No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT52490.11780749236798671No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT52480.11778504857062187No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT52260.11729128502859563No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC51440.11545089364467967No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT51360.11527134326576104No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG50060.11235364960833329No Hit
CCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCG49770.11170277948475324No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAG49520.11114168455063254No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT49400.11087235898225459No Hit
TTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGAC48850.10963795012718901No Hit
TAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTT48520.10889730481414965No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA48450.10874019823259584No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG48300.1084035412721234No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA48250.10829132228529925No Hit
GTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT47550.10672025646976124No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC47470.10654070609084261No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG46900.10526140964104737No Hit
AAGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCT46470.10429632635435973No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG46060.10337613066240174No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA46020.10328635547294243No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT45070.10115419472328369No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT45050.10110930712855404No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA45030.10106441953382439No Hit
TCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCT45000.10099708814172989No Hit
GAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGG44740.10041354941024434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)