FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_G2_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_G2_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5580458
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC621181.1131344416533553No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG504400.9038684638429318No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA424420.7605468941796534No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT399710.7162673744699808No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA350650.6283534433912055No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT327850.5874965818217788No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG293930.526713040399193No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT248610.44550106819189395No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA236090.42306563368096306No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA233100.41770765051900755No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC227650.40794142702982444No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG226630.4061136200648764No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA213210.3820654147025208No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG207130.3711702516173404No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA204560.3665648948527164No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA197410.3537523264219532No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA194490.3485197809928863No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC190440.3412623121614749No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT188180.3372124653567861No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC186690.33454243361387187No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC184580.3307613819510872No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC178590.3200274959510492No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA174700.3130567419376689No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC174250.31225035651195654No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT171550.30741204395768235No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA170950.30633686339006583No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA163030.2921444798975281No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT159990.2866968983549379No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA159910.2865535409459224No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA159570.28594427195760636No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT157630.28246785478897973No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA155600.2788301605352106No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT155470.2785972047455603No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC153250.2746190366453793No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC151410.27132181623802204No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC150000.2687951419041233No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT149520.2679349974500301No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG145640.2609821631127768No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG144180.2583658903982433No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT140050.25096506415781644No Hit
GAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGA136510.24462149880887912No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA135770.24329544277548548No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA135490.2427936918439311No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC135340.24252489670202695No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA135250.2423636196168845No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG132020.23657556422788237No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA130350.23358297831468314No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC125520.22492777474537035No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC123940.2220964659173136No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA123570.22143343790061676No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT123330.22100336567357015No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA123260.22087792794068156No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA122790.2200357031627153No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG121560.21783158299910152No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG118860.2129932704448273No Hit
ATGCTGGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGG117670.21086082898572125No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC117400.21037699773029384No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA114270.20476813910256111No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG113710.20376463723945237No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA113230.2029044927853592No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG112420.20145299901907693No Hit
CTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCC112230.20111252517266504No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA111370.19957143302574806No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC110320.1976898670324192No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA108670.19473312047147384No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT108090.19369377925611123No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGA107750.19308451026779525No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA107410.1924752412794792No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG107050.19183013293890933No Hit
GAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGG106160.1902352817636115No Hit
GGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTC106000.18994856694558046No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA105910.18978728986043797No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC105740.18948265536627998No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT105640.18930345860501055No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT105440.18894506508247172No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG104770.18774444678196664No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA103790.18598831852152636No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTT103580.1856120053228606No Hit
ATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTC101620.18209974880198004No Hit
CCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCA100640.1803436205415398No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA100030.17925052029779634No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT98200.17597121956656603No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG98130.17584578183367747No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG96680.17324742879527094No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC96630.17315783041463623No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC96450.17283527624435127No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA94090.16860623267839306No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTG93780.1680507227184579No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC93600.1677281685481729No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA93500.1675489717869035No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA91050.16315865113580283No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCA90890.16287193631777178No Hit
TGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTC90760.1626389805281215No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG90140.16152796060825114No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC88250.15814114182025918No Hit
CTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCA87640.15704804157651578No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA86040.1541808933962051No Hit
CTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT85160.15260396189703426No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA84510.15143918294878306No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA84290.15104495007399035No Hit
GGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGG83660.14991601047799302No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC83410.14946801857481948No Hit
CTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT81520.14608119978682754No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCA80160.14364412383356348No Hit
GGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGC78830.14126080690868026No Hit
CCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGT77970.13971971476176326No Hit
GTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGT77560.13898500804055866No Hit
GAACAAGGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCAGACCG77520.1389133293360509No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG76670.13739015686526088No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC76560.1371930404278645No Hit
CAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG75920.13604618115574024No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA75790.13581322536609003No Hit
CTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGG75190.13473804479847354No Hit
ATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTA74930.13427213321917306No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT74050.1326952017200022No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA73060.13092115378343497No Hit
GGCAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGC72470.12986389289194544No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA72000.12902166811397917No Hit
TGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGT71960.1289499894094714No Hit
CAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCAT71930.12889623038109058No Hit
CAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAA71400.12794648754636267No Hit
GAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCT71030.12728345952966583No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG70860.12697882503550784No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA70850.1269609053593809No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAAC70280.12593948382014522No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG70280.12593948382014522No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT69550.1246313474628785No Hit
GTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCC69450.12445215070160907No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT68340.12246306665151857No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT67910.12169252057806008No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC67730.12136996640777513No Hit
CTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCT67210.12043814324917416No Hit
ATCCAGATGAAGGCACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTT66380.11895081013063803No Hit
GGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCAGACCGAGTGAT66140.11852073790359141No Hit
GCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGA65940.1181623443810526No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT65560.11748139668822882No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT65550.11746347701210187No Hit
CTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATT65240.11690796705216668No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA65140.11672877029089727No Hit
TGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAAC64700.11594030454131184No Hit
CACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTT64590.11574318810391548No Hit
GACTTACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGAT63600.11396914016734827No Hit
GGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCA63560.11389746146284051No Hit
GATGAAGGCACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCAT63400.11361074664480944No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG62850.11262516445782766No Hit
GCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTA62730.11241012834430435No Hit
TTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATC61550.11029560656132524No Hit
GTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCT61480.11017016882843667No Hit
ACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTT61460.11013432947618279No Hit
GTGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAG61320.1098834540104056No Hit
GGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATG61130.10954298016399372No Hit
GTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATT60870.10907706858469322No Hit
ACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATT60710.10879035376666216No Hit
AGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGA60510.10843196024412333No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA59720.10701630583009494No Hit
ACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCA59130.1059590449386054No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA59090.10588736623409763No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA58800.10536769562641632No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA58740.10526017756965467No Hit
CTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGA58530.1048838643709889No Hit
GCTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGG58190.10427459538267289No Hit
TCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTG57810.1035936476898491No Hit
CAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTG57720.10343237060470664No Hit
GTATCACCTCTGGCCGTAACATGGTGGAATAGGAATGGCCCAACAACAAG56960.10207047521905907No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA56100.10052938307214211No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA56070.10047562404376129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)