FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_F1_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_F1_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147148
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC12330.8379318781091147No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG9560.6496860303911708No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT8160.5545437246853508No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA7650.5198847418925164No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG7510.5103705113219343No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA6860.466197297958518No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA6740.4580422431837334No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT6150.41794655720770924No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA4680.31804713621659825No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA4660.31668796042080083No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT4640.3153287846250034No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA4630.3146491967271047No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG4620.313969608829206No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC4580.3112512572376111No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC4480.30445537825862395No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC4240.2881452687090548No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA4230.28746568081115614No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA4190.28474732921956125No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC4140.2813493897300677No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT3880.26368010438470113No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA3860.2623209285889037No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG3770.25620463750781525No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT3750.25484546171201783No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA3750.25484546171201783No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA3650.24804958273303068No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC3510.2385353521624487No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC3430.233098648979259No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC3380.22970070948976543No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT3370.2290211215918667No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA3370.2290211215918667No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG3360.22834153369396798No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG3310.2249435942044744No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT3260.22154565471498083No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA3230.2195068910212847No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC3210.21814771522548726No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC3170.21542936363389242No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT3100.2106722483486014No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC3030.2059151330633104No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC3030.2059151330633104No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT3030.2059151330633104No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA3020.2052355451654117No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA3010.20455595726751297No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG2970.20183760567591813No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA2970.20183760567591813No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA2950.20047842988012068No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA2950.20047842988012068No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC2870.19504172669693098No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA2850.19368255090113357No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA2820.1916437872074374No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC2780.18892543561584257No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT2780.18892543561584257No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGA2740.18620708402424768No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2700.18348873243265285No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC2670.1814499687389567No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG2650.18009079294315927No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA2640.17941120504526054No Hit
GAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGA2610.1773724413515644No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA2590.17601326555576696No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA2590.17601326555576696No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG2580.17533367765786828No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT2550.17329491396417213No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT2540.17261532606627342No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA2530.1719357381683747No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTG2450.16649903498518498No Hit
GGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTC2430.16513985918938756No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA2380.161741919699894No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG2380.161741919699894No Hit
ATGCTGGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGG2370.16106233180199525No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC2330.15834398021040041No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG2330.15834398021040041No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC2300.15630521651670426No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC2290.15562562861880555No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA2280.15494604072090684No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG2260.15358686492510942No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA2260.15358686492510942No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT2250.1529072770272107No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT2240.15222768912931198No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT2230.15154810123141327No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT2230.15154810123141327No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA2190.14882974963981843No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC2160.14679098594612228No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA2140.14543181015032486No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA2130.14475222225242612No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC2130.14475222225242612No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT2100.14271345855873No Hit
GAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGG2100.14271345855873No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA2080.14135428276293255No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG2050.13931551906923642No Hit
GGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGG2030.13795634327343897No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA2020.13727675537554027No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG2010.13659716747764156No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG2010.13659716747764156No Hit
CCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCA2010.13659716747764156No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC2010.13659716747764156No Hit
CTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCC2000.13591757957974285No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG1990.13523799168184414No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA1970.1338788158860467No Hit
GGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCAGACCGAGTGAT1950.13251964009024927No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA1950.13251964009024927No Hit
TTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATC1940.13184005219235057No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA1870.12708293690705955No Hit
GAACAAGGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCAGACCG1830.1243645853154647No Hit
GTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGT1830.1243645853154647No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTT1830.1243645853154647No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCA1800.12232582162176855No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA1800.12232582162176855No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA1790.12164623372386985No Hit
ATCCAGATGAAGGCACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTT1780.12096664582597114No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG1750.11892788213227498No Hit
CAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCAT1720.11688911843857885No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA1700.11552994264278141No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCA1700.11552994264278141No Hit
GTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCC1700.11552994264278141No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC1690.11485035474488271No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA1680.11417076684698399No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG1670.11349117894908528No Hit
GGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGC1620.11009323945959171No Hit
TGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAAC1620.11009323945959171No Hit
GGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCA1590.10805447576589557No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT1580.10737488786799684No Hit
TGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTC1560.10601571207219943No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA1520.10329736048060457No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT1510.10261777258270585No Hit
CCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGT1510.10261777258270585No Hit
CTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT1500.10193818468480713No Hit
ATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTC1500.10193818468480713No Hit
TGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGAC1490.10125859678690842No Hit
CAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGGAGTCT1480.1005790088890097No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)