FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_E8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_E8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA60.4784688995215311No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG40.3189792663476874No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA40.3189792663476874No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC40.3189792663476874No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.23923444976076555No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC30.23923444976076555No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.23923444976076555No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.23923444976076555No Hit
AAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGT30.23923444976076555No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG30.23923444976076555No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA30.23923444976076555No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC30.23923444976076555No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.23923444976076555No Hit
TGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGGAGGTTGCTAATCCT30.23923444976076555No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC30.23923444976076555No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA30.23923444976076555No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC30.23923444976076555No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG30.23923444976076555No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG20.1594896331738437No Hit
GGAGAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCAGAGCAGAAA20.1594896331738437No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACCTGTCTCTTA20.1594896331738437No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA20.1594896331738437No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTTCTG20.1594896331738437No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA20.1594896331738437No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGA20.1594896331738437No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT20.1594896331738437No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA20.1594896331738437No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA20.1594896331738437No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGCTGTCTCTT20.1594896331738437No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA20.1594896331738437No Hit
CAAATAACATTTATGCAAGCCTTACAACTACTGCTTGAAGTAGAACAAGA20.1594896331738437No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA20.1594896331738437No Hit
AAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGATACTACTA20.1594896331738437No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCTGTCTCTTATAC20.1594896331738437No Hit
CCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGT20.1594896331738437No Hit
GGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCA20.1594896331738437No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA20.1594896331738437No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC20.1594896331738437No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC20.1594896331738437No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA20.1594896331738437No Hit
GTTGTAATTGAATACTGGAGAATTGCACCTTTCGACCTTTTCGAAATAAG20.1594896331738437No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT20.1594896331738437No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA20.1594896331738437No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA20.1594896331738437No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC20.1594896331738437No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC20.1594896331738437No Hit
CTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTC20.1594896331738437No Hit
GAGTGGCTGTTTCGATATCGAGGCCAAGGGTGTTGCCTCTTCCTTTTAAG20.1594896331738437No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA20.1594896331738437No Hit
AGAAGGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTGAAGCT20.1594896331738437No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG20.1594896331738437No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA20.1594896331738437No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA20.1594896331738437No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATTGGGCA20.1594896331738437No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC20.1594896331738437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)