FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_E4_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_E4_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATACTTTTGGT12.5No Hit
CACAAAAACAAACACACAAAAACAAACAAAAAAAAAAACACACACAAAAA12.5No Hit
GAAGACAAGAGATATGGCCCAGAATTAAGCATCAATGAACTGAGCAATAT12.5No Hit
ATATTTGTTTTTACATAGATATTCCAAAATTTTACTCCAAGAGCTTAAAC12.5No Hit
ATACACAATTACAGAATACAAGAGAATAATATGGCCCAGCATTAAGCATC12.5No Hit
AAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACA12.5No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTAAGTCC12.5No Hit
GTCTGTGACGGGACCACAACACACACCCAGACCCGGGTGCCGTCAGAGAC12.5No Hit
ATCAATAAGTATACCAATAGTTTAAAAAAGACCTTCTTTCTGTCACTTAT12.5No Hit
GGGCAGAGGGAGAGGAAAACAATCCTCAAGCAGGGAGCCTGGTGCGAGGC12.5No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG12.5No Hit
GCCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTT12.5No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTGTTCGTTTCATTACC12.5No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT12.5No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC12.5No Hit
ACCTTGACCAATTAGCACATTAGCGTTCTCTCCTATTGAAAGATTGCTAA12.5No Hit
CAATTGTGCAATAGATACCAAGGTGCAGGGATCATGACCTCAGGGGAAGG12.5No Hit
CAAAAAATCAACAACTTCATCAAATAAGAAGCCCTGTCTATTAAAAACAT12.5No Hit
GAGCAAAAGCAGGGACTTTTGGGCAGCATAAAGATGTATGAGCTGAATTC12.5No Hit
TTACCAAAACAACGTCCCCTTTCAAAATTAGAAAATTAGCCTTCTCTACT12.5No Hit
CTTTTGACGACTCACTATAGGGAGTAGGAACAAGGTCTGTCTCTTATACA12.5No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGAAAACAAAATGATGGCAAT12.5No Hit
TACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACA12.5No Hit
GGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGG12.5No Hit
GACAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCCATGAGATACCC12.5No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT12.5No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA12.5No Hit
TAAATAAACTGAGCAATCTTGCAAAAGTAGAGAACGATAATGTGCTAATT12.5No Hit
ATAATCCGCCATAAAAATAGTCAAATTTGCCAAAAAATAAAAATCCTCTC12.5No Hit
AGTAAAGTTATATTAACAAAACAAACAAAAACTACAAATTACCAAATATA12.5No Hit
TATAAAAACAACGCAAAACAAATGAATGGATTTCAATAAGAATATACTTT12.5No Hit
ATATGATACACAACCAAATTCCAATACAAACAAAAAAAAAGAAAACATAA12.5No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG12.5No Hit
CATTAGAGATAACTCATATACCAACATACCACAAAACAATGATCATTAAA12.5No Hit
CTGTAGACAATATGGCCAAAATCAAAAACTACACATCAGGAAGGAAAGAG12.5No Hit
CTACATTAACAAAAACTAATAAATACAAAAACAACCTCAACTTCAACAAT12.5No Hit
CAATGATATTTGCTCAAAAGACTACAGGATCACTTCAATAGATGCATCTG12.5No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT12.5No Hit
CTATTCGACAACTAATTTATGGCCATCAGAATTCTTTAGGTCGCTGTCTG12.5No Hit
AAAATGACCACATCATTCACAAATTTATGATCATTTTGTTTTTGCTACAA12.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)