FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_E3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_E3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC90.40286481647269473No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA90.40286481647269473No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG70.3133393017009848No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT60.26857654431512984No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA60.26857654431512984No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT50.22381378692927484No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA50.22381378692927484No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT50.22381378692927484No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT40.17905102954341987No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA40.17905102954341987No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG40.17905102954341987No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC40.17905102954341987No Hit
TCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTG40.17905102954341987No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA30.13428827215756492No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA30.13428827215756492No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.13428827215756492No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC30.13428827215756492No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC30.13428827215756492No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA30.13428827215756492No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCTGTCT30.13428827215756492No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.13428827215756492No Hit
CCAGAGGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTC30.13428827215756492No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA30.13428827215756492No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC30.13428827215756492No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC30.13428827215756492No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG30.13428827215756492No Hit
GTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGT30.13428827215756492No Hit
TCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGG30.13428827215756492No Hit
GCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTA30.13428827215756492No Hit
TTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTC30.13428827215756492No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC30.13428827215756492No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA30.13428827215756492No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC30.13428827215756492No Hit
AATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACG30.13428827215756492No Hit
CTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT30.13428827215756492No Hit
CTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCA30.13428827215756492No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA30.13428827215756492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)