FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_C7_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_C7_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7288064
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC467430.641363742140574No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA458150.6286305938037866No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG455250.6246514849485405No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC405680.5566361656538691No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA372500.511109671923847No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT331510.4548670264146967No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA314750.4318705214443781No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT286330.39287525466296674No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC282760.38797683445150866No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA275380.3778506884681584No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA255330.3503399531068882No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC218380.2996406178650462No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC216370.29688268379641014No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA200660.275326890652991No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC198550.272431745934174No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA198520.27239058273911976No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT198410.2722396510239207No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT189980.260672793213671No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC187150.2567897318135516No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA186980.256556473708244No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA185730.25484134058098284No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT183000.2510954898310443No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG175710.2410928334328568No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA174290.23914444220028805No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG172610.23683930327724895No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA154220.21160626470898172No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT139120.19088745653166603No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG138160.1895702342899294No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT135830.18637322614071444No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC130700.17933431978643435No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA127380.17477892620042854No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC126120.1730500720081492No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG124730.17114284397063473No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC123560.1695374793635182No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA118980.16325323158523306No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC118770.16296508921985317No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA118560.1626769468544733No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT115820.15891737503951667No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA115200.1580666690083951No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT114090.1565436307913871No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT113530.1557752511503741No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT112010.15368964926762443No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT111380.15282522217148478No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG109370.15006728810284872No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT107740.14783075450490007No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA104560.1434674558291475No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC103820.14245209701780884No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG102660.1408604534757104No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC102230.14027044767993255No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC98740.1354817959886192No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA98470.1351113272331308No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA96280.1321064139941691No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA95480.1310087287927219No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG95460.13098128666268574No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC93690.12855265815448383No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA93640.12848405282939337No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC93240.1279352102286698No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC92130.1264121720116618No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA91880.12606914538620956No Hit
CAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG90340.12395610137342372No Hit
CTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCA90160.12370912220309811No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA89630.12298190575713934No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC89190.12237817889634338No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA87330.11982606080297867No Hit
GTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATT86520.11871465453651339No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA86140.11819325406582598No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA85640.1175072008149215No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG85260.11698580034423407No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG84920.11651928413361902No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA84770.11631346815834769No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA84370.11576462555762407No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA83820.11500996698162913No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA83200.11415926095050756No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA81780.11221086971793881No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA81660.11204621693772174No Hit
GTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCT81530.11186784309248655No Hit
ATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTC81350.11162086392216095No Hit
TCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTG80960.11108574238645544No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA80740.11078387895605746No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA79370.10890409304857916No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA78080.10713407566124557No Hit
TGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTC77730.10665383838561243No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA77130.10583057448452704No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC75530.10363520408163265No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA74000.10153588113386491No Hit
GAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAACGACCTTG73860.10134378622361165No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTCCTGTCTCT73350.10064401190768907No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)