FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_B6_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_B6_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6054295
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA567040.9365912959312356No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTTCTGTTTACTGTGT438390.7240975208509001No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA276960.4574603649144946No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC274700.4537274777657844No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG250580.41388799191317893No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG225160.37190127009007656No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA224380.3706129285077784No Hit
GGATACACCATGGACACAGTAAACAGAACATTCGAGAAATTTTTCCCTAG210570.34780267562119127No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG197380.32601648912053344No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT193960.32036760679814913No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTTCTGTTTACTGT192790.3184350944247018No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT192700.3182864396267443No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA191630.31651909925102756No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG186810.3085578089604157No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA171870.2838811124994735No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG170430.2815026357321538No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT160200.2646055403643199No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA158270.26141772080812054No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG153910.2542162217070691No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC144590.2388221915185831No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC138860.22935783604862334No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCAGTCTTCTGTC130050.21480618304856305No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA129210.21341873826762653No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA129110.213253566269896No Hit
GTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAG127620.210792503503711No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC126490.20892605992935595No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA123490.20397089999743984No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT121430.2005683568441908No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT121330.20040318484646022No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA118800.19622433330387765No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC115770.1912196217726424No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC115740.19117007017332324No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT114820.1896504877942023No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG112570.18593411784526523No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATAAGATCTGTT112370.18560377384980414No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT109400.1806981655172072No Hit
ACCATGGACACAGTAAACAGAACATTCGAGAAATTTTTCCCTAGCAGTTC105780.17471893919936177No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA105230.17381049321184383No Hit
AATGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCT103890.17159718844225463No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA103830.17149808524361632No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA103720.17131639604611273No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATAAGATCTGTTCCACCATTGA102520.16933433207334628No Hit
CTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGG101890.1682937484876439No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG99330.16406534534574216No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG98880.16332207135595475No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT96690.15970480460565598No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA95870.15835039422426558No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA94900.15674822584627937No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC93240.15400637068395245No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA90860.1500752771379657No Hit
CATAAGATCTGTTCCACCATTGAAGAACTCAGACGGCAAAAATAATGAAT90590.14962931274409325No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT89300.1474985939733693No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA87820.14505404840695738No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACATTCGAGAAA87390.14434380881671607No Hit
GTCCCTAAGTGCCTGAACAATGTTTTCAAAGAATTCCTTGGTCATGTCTT85020.14042923247050235No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC83680.13821592770091315No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA81620.1348133845476641No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC81610.13479686734789104No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC78790.1301390170118899No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC77970.1287846066304995No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT77910.1286855034318612No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG77350.12776054024457018No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT77040.1272485070516055No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACATTCGAGAAATTTTTC76240.12592713106976122No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT75860.12529947747838519No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT75510.1247213754863283No Hit
GTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTA75460.12463878948746304No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT75430.12458923788814388No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG74900.12371382630017203No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC73740.1217978311264978No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC72740.12014611114919242No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG72560.11984880155327747No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG72220.11928721676099363No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT71280.1177345999823266No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG71190.11758594518436911No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT70640.11667749919685116No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG70620.11664446479730506No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT70320.11614894880411343No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA69880.11542219201409908No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT69170.11424947083021227No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG68150.11256471645336079No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA67560.11159020166675064No Hit
GTATGGAGGATCTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCG67480.1114580640685662No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC67280.11112772007310513No Hit
CTTCAATGGTGGAACAGATCTTATGATCTCAGAGAACTCTTCTTTCTTGA67090.11081389327741711No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTTCTGTTTACT66280.10947600009579975No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG65710.1085345197087357No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA65110.10754348772235248No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC64030.10575963014686268No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC63560.10498332175752915No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC63520.10491725295843696No Hit
GTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAG63500.10488421855889082No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG63410.10473556376093336No Hit
CTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTT63300.10455387456342977No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATAAGATCCTGT63210.10440521976547229No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC62920.10392622097205372No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT62350.10298474058498966No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT62240.10280305138748608No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT61470.10153122700496094No Hit
ATATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC61160.10101919381199627No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG61030.10080447021494658No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCTGT61020.10078795301517351No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA60890.10057322941812383No Hit
CTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCA60800.10042457462016634No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC60700.10025940262243581No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)