FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_B5_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_B5_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC40.3883495145631068No Hit
ACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCC40.3883495145631068No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA30.2912621359223301No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT30.2912621359223301No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC30.2912621359223301No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.2912621359223301No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC30.2912621359223301No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.2912621359223301No Hit
CAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACA30.2912621359223301No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA30.2912621359223301No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG30.2912621359223301No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA30.2912621359223301No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG20.1941747572815534No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT20.1941747572815534No Hit
TGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCG20.1941747572815534No Hit
ACCCAATCCATTGTCTGCAAATCGCTTGCGGATATGCCAAAGGAAACAGT20.1941747572815534No Hit
ACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAG20.1941747572815534No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGCTGTCTCTTATACAC20.1941747572815534No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG20.1941747572815534No Hit
TACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACT20.1941747572815534No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCTGTCTCTTATAC20.1941747572815534No Hit
GGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCT20.1941747572815534No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA20.1941747572815534No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA20.1941747572815534No Hit
GTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCC20.1941747572815534No Hit
GTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGGAGTCTGCT20.1941747572815534No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACTTCCCCTGTCTC20.1941747572815534No Hit
TCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGA20.1941747572815534No Hit
GCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGA20.1941747572815534No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA20.1941747572815534No Hit
GAGTGGGTGGGGCTCGGAGTGCCGAGAAGTGAAGGCGACTGCCCAGCGAG20.1941747572815534No Hit
ACTGTGATGAGAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAA20.1941747572815534No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC20.1941747572815534No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA20.1941747572815534No Hit
CATTGTCTGCAAATCGCTTGCGGATATGCCAAAGGAAACAGTCTACCTGA20.1941747572815534No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG20.1941747572815534No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT20.1941747572815534No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA20.1941747572815534No Hit
GGAATGGATCTTGAAAGAGGAATCCAGCGAGACACTTAGAATGACAATTG20.1941747572815534No Hit
ATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT20.1941747572815534No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20.1941747572815534No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG20.1941747572815534No Hit
GGCCAAGGGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGC20.1941747572815534No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC20.1941747572815534No Hit
GAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAG20.1941747572815534No Hit
GTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAG20.1941747572815534No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC20.1941747572815534No Hit
GCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACT20.1941747572815534No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC20.1941747572815534No Hit
CTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT20.1941747572815534No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT20.1941747572815534No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG20.1941747572815534No Hit
TTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCCAGC20.1941747572815534No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA20.1941747572815534No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC20.1941747572815534No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC20.1941747572815534No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT20.1941747572815534No Hit
ACCTTCTCTTCCAGGACATACTTATGAGGATGTCAAAAATCTGTCTCTTA20.1941747572815534No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG20.1941747572815534No Hit
CTTCAGTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTT20.1941747572815534No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT20.1941747572815534No Hit
GTTAACTGAAGAGTGGATGAGAAAGGGTAGGATAGGACAAGGCAGACACC20.1941747572815534No Hit
GTGGATTGGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCA20.1941747572815534No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT20.1941747572815534No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT20.1941747572815534No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG20.1941747572815534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)