FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_A4_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_A4_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3261167
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA347301.0649561951289215No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATGACGGATCAAGAA209210.6415188182635234No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT186740.5726171030186433No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG165220.5066284553964884No Hit
CAGTAAACAGAACACACCAATGACGGATCAAGAAAGAAGAGTTCTCTGAG160610.4924924114588428No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA150210.46060198695742965No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG130770.4009914242355574No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT127080.38967645631149833No Hit
CTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGG119050.3650533689320418No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCATTGGTGTG118990.3648693857137644No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG116610.3575713847220949No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG116260.35649814928214346No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCATTGGTGTGTTCTGTTTAC107170.32862469171311987No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC98630.3024377469783056No Hit
GTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAG94850.29084680422683046No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT94380.2894056023503243No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCATTG94250.2890069720440566No Hit
TCATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTC94170.2887616610863535No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATGA86460.26511981753770963No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT81060.24856132789274513No Hit
ACCATGGACACAGTAAACAGAACACACCAATGACGGATCAAGAAAGAAGA78060.23936216697887594No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC77460.23752233479610213No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG76820.2355598471344767No Hit
CTTCTTTCTTGATCCGTCATTGGTGTGTTCTGTTTACTGTGTCCATGGTG75730.23221748533577088No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA73430.22516479530180455No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT71800.22016658453860227No Hit
AAACAGAACACACCAATGACGGATCAAGAAAGAAGAGTTCTCTGAGATCA71120.21808144139812527No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA68820.2110287513641589No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCA67970.20842232243856265No Hit
GTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTA67590.20725709538947254No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA66960.20532527159756003No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC65970.20228954849598316No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA64130.1966473964688101No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA63980.19618743842311662No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG63520.19477690041632337No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT63500.19471557267689757No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACACACCAATGACGGATC63060.1933663624095301No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC60150.184443176323077No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC59660.18294064670714502No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC58040.17797309981365567No Hit
CACCAATGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTG55420.1699391659488766No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG54970.1685592918117962No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT54950.16849796407237042No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT53030.16261050108749414No Hit
GTATGGAGGATCTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCG51820.15890017285223357No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC50290.15420860078616028No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA50120.15368731500104105No Hit
TTCTTGATCCGTCATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCC49450.15163283573027692No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA48150.14764653266760028No Hit
CACCATGGACACAGTAAACAGAACACACCAATGACGGATCAAGAAAGAAG48110.14752387718874868No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT48060.1473705578401842No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT47790.146542633357936No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA47680.1462053307910941No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG46460.14246433868612066No Hit
CCATGGACACAGTAAACAGAACACACCAATGACGGATCAAGAAAGAAGAG44330.13593293443727353No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT44050.1350743460853124No Hit
ATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCG44030.1350130183458866No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA43310.132805219726558No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA42010.12881891666388134No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT41810.12820563926962342No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA41170.126243151607998No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT40760.1249859329497692No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG40440.1240046891189565No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT40070.1228701259395793No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC40000.122655478851589No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT39680.1216742350207763No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG39310.12053967184139912No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT39220.12026369701398303No Hit
GAACACACCAATGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAG39180.12014104153513146No Hit
CTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTT39070.11980373896828957No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC38660.11854652031006079No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC38400.11774925969752546No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT37950.11636938556044507No Hit
GGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGG37700.11560278881762266No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC37560.11517349464164209No Hit
ACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATT37170.11397760372283908No Hit
GTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG36810.11287370441317478No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA36750.11268972119489742No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA36620.11229109088862974No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA36560.11210710767035237No Hit
GCTGTATGGAGGATCTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTT36420.1116778134943718No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT36390.11158582188523311No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC36120.11075789740298489No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC36040.11051258644528171No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC35740.10959267035389479No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT35320.1083047878259531No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT35140.10775283817112094No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC35050.10747686334370488No Hit
TTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA34950.1071702246465759No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG34710.10643429177346636No Hit
GTCATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTT34640.1062196446854761No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC34520.10585167824892132No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG34490.10575968663978264No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC34020.10431848476327645No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT33970.10416516541471198No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG33080.10143608101026412No Hit
GGACACAGTAAACAGAACACACCAATGACGGATCAAGAAAGAAGAGTTCT33060.10137475327083832No Hit

[FAIL]Adapter Content

Adapter graph