FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_A2_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P2_A2_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5157422
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC642391.2455641597681941No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG553411.073036102145607No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA436460.846275522925989No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA426110.8262073570865444No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT389820.7558427446891102No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT369140.715745192074645No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG255510.49542193754941904No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC246650.478242812009566No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA209850.4068893334693186No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG208600.4044656419428156No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT204210.39595363730173716No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA200690.3891285219631048No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT199570.3869568943553581No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA194070.3762926516387451No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA190310.3690021875270242No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA188160.3648334381014391No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC182100.35308338158095265No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG179960.3489340216875796No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT179330.34771248115822206No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC178760.34660727782213674No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT173240.3359042560410996No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC169050.32778004204426164No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA168910.3275085885932933No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA168870.3274310304644452No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA167760.3252787923889106No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA164450.31886085722673074No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC164170.31831795032479404No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT163480.31698007260216443No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC160680.31155100358279775No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT155630.30175928981572575No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC154710.2999754528522196No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG153640.2979007729055331No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC152440.29557402904009017No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC150950.29268498874049864No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA140710.27283010775538635No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT140440.2723065903856617No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC139400.2702900790356112No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA138580.26870013739422527No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA133680.2591992666103336No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT131760.25547647642562504No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG127260.24675118693021436No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC126970.24618889049606565No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA126970.24618889049606565No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA126770.24580109985182522No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC126740.24574293125518912No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC124400.24120578071757554No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA123930.24029447270361046No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA121470.23552464777945262No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA118240.22926182887496893No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA116800.2264697362364375No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA113010.2191211035280805No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC111390.21597999930973266No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA110120.21351752871880564No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA109260.2118500289485716No Hit
ATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTC108470.2103182559038217No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT108000.2094069478898566No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG106670.20682814010565742No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA106060.205645378640724No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTT102960.19963462365499662No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG101810.1974048274506139No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA100080.1940504383779338No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG99040.1920339270278833No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA99020.19199514796345926No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA98620.19121956667497833No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA98370.1907348283696777No Hit
GAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGA98100.1902113109999531No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG97900.18982352035571262No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG97710.18945511924368416No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC97330.18871831701962724No Hit
TGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTC96420.18695386958833307No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG95240.1846659047873143No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC94940.18408421882095358No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT94420.18307596314592833No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT94270.18278512016274798No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA93950.18216465513196323No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC93560.18140846337569427No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG93530.18135029477905823No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA93110.1805359344261532No Hit
CTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCA92740.17981852173430835No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA92660.17966340547661216No Hit
GAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGG91660.17772445225540978No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA90960.1763671850005681No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG90710.17588244669526754No Hit
CCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCA89370.17328424937885634No Hit
GGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTC88200.17101567411004956No Hit
CAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG88000.17062788346580907No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCA87840.1703176509504167No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA87070.16882465697009086No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT83910.16269756479109138No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG82270.15951768150831946No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGA81110.1572684957717247No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC80630.15633779822554755No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT80410.15591122851688305No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA76650.14862076440516211No Hit
ATGCTGGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGG76490.14831053188976973No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA76320.14798090984216533No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG76050.1474573924724407No Hit
CTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCC75870.14710838089262426No Hit
CTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT74770.14497553234930166No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA74370.1441999510608207No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT74200.1438703290132163No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC74080.143637654626672No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC72350.1402832655539919No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG72300.14018631789293176No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA72080.13975974818426726No Hit
GGCAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGC72020.13964341099099511No Hit
CTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT71720.1390617250246344No Hit
GAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCT69290.13435006869711263No Hit
CTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGG69120.1340204466495082No Hit
ATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTA68990.13376838273075192No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC68510.13283768518457478No Hit
CTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATT67860.13157736559079322No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCA67790.13144163886530907No Hit
CAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAA67100.13010376114267944No Hit
GTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCT65730.12744739522963217No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT65480.12696265692433156No Hit
GTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATT65450.1269044883276955No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC65280.1265748662800911No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA65030.1260901279747905No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA63980.12405422709252802No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT63310.12275512843432242No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA62120.12044777410109159No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA62050.12031204737560743No Hit
GGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATG62040.12029265784339539No Hit
TGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGT61970.12015693111791124No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA61250.11876088479864554No Hit
TCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTG61120.11850882087988922No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT61060.11839248368661708No Hit
CCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGT60370.11705460596398744No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAAC58940.11428190285766802No Hit
GATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCT58620.11366143782688327No Hit
GTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCC58170.11278890887734219No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTG57540.11156736834798471No Hit
AGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGA57480.11145103115471258No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG56900.1103264382864152No Hit
CTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATC55880.10834870600078877No Hit
GGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGC55670.10794152582433628No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA55600.1078057990988521No Hit
ACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTT55540.10768946190557996No Hit
GGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGC55210.10704960734258318No Hit
GAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAA55190.10701082827815912No Hit
ACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCA55000.10664242716613068No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG54900.10644853184401043No Hit
GCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTA54790.10623524698967818No Hit
CACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTT54510.1056923400877415No Hit
GTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGT54430.10553722383004531No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT53770.10425751470405176No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA53210.10317170090017842No Hit
GTGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAG52580.10195016037082091No Hit
GCTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGG52210.10123274767897604No Hit
CTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCT52200.10121335814676402No Hit
ACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATT52110.10103885235685582No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA51660.10016632340731474No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG51600.10004998621404261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)