FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_G8_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_G8_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6214
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC330.531058899259736No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT290.4666881235918893No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG240.3862246540070808No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT190.3057611844222723No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT160.2574831026713872No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG160.2574831026713872No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA130.20920502092050208No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA130.20920502092050208No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA120.1931123270035404No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA110.1770196330865787No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG100.16092693916961698No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG100.16092693916961698No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC100.16092693916961698No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC100.16092693916961698No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC90.14483424525265529No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC90.14483424525265529No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT90.14483424525265529No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC90.14483424525265529No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC90.14483424525265529No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT90.14483424525265529No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC90.14483424525265529No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA90.14483424525265529No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC90.14483424525265529No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC90.14483424525265529No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC90.14483424525265529No Hit
GGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAA80.1287415513356936No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA80.1287415513356936No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG80.1287415513356936No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA80.1287415513356936No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT80.1287415513356936No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA70.11264885741873189No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG70.11264885741873189No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA70.11264885741873189No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT70.11264885741873189No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC70.11264885741873189No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT70.11264885741873189No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA70.11264885741873189No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT70.11264885741873189No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA70.11264885741873189No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC70.11264885741873189No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT70.11264885741873189No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA70.11264885741873189No Hit
GTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATT70.11264885741873189No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA70.11264885741873189No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG70.11264885741873189No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT70.11264885741873189No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT70.11264885741873189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)