FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_F4_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_F4_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCGATTCACAGCATCGGTCTCACAGACAGATGGCTACTACCACCAAT11.3513513513513513No Hit
TGCCACCCACCCCAACCACCCCCCCCCCCGCCACCCCCCCCCCCACCCCG11.3513513513513513No Hit
GACTAGACTTATTACAAACATCAAATGCCTCCCATAAATTAACCCACGAT11.3513513513513513No Hit
CAGATTCAACAAGGGGAGTGCAGTAATGCTCACATGCCAAAGATTGAAGC11.3513513513513513No Hit
CCTTCTCAACCACCACAAACAAAACAGGATGTCAAAAATGCACTTGCCCA11.3513513513513513No Hit
ACTGTGATGAGAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAA11.3513513513513513No Hit
GTCTGATAGCGGCCCGTTTACTTTTCACTAACAACACTACGACACCTAGC11.3513513513513513No Hit
GAATCATAACCAACAACAACATCAAACAAAGTGAACTAATAAAAACATAC11.3513513513513513No Hit
TCACAAAGGAGGGACCAATAGTTGGAGAAATTTCAACATTACCTAATAAT11.3513513513513513No Hit
ACACATAAGTATGTCCTCAAAAACAATGAAAAAGTGAAAAAAATAAAACT11.3513513513513513No Hit
GAGCAAAACCAGCCCAAAACAAAACCAACAAAAAACAACCCAATACAACA11.3513513513513513No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT11.3513513513513513No Hit
ATCTTAAGAACTCAGAGAACTCAACTTTCTTGATCCCACCAGACACGAAC11.3513513513513513No Hit
ATATACAACACTAATCAAATGAAAAGAATTCTAATTTTCTCAAAAGAAAA11.3513513513513513No Hit
CGCTTCAGGTAGGCATGCAAAATTACAAGAAGGTCATCTTTCAGAAAAGC11.3513513513513513No Hit
CTACTCTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC11.3513513513513513No Hit
CCACAAGACGGAAAAAACATGTTCATTCATATCTGGAAACAACTCCATTA11.3513513513513513No Hit
GGATGAACAGATCTACCAGAACAGCAGCAATCAAAACCAGAAATAATTCC11.3513513513513513No Hit
CTCAAACACAATGTCAAGCTATCACTTATAAAGATTCCTTTGGAATAAAC11.3513513513513513No Hit
AATCTTATACGTCCGCAAAACCTTATCTAAGTAATCTTGGCTAATCAAAA11.3513513513513513No Hit
TTCATACTGTGTCTACAGTGACTGTTGAATTGTTAGCATGATAACCTATA11.3513513513513513No Hit
AGATGGCTCTTCAATTGTTCATCAAAGACTACAGATACACATATAGGTGC11.3513513513513513No Hit
CATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCAC11.3513513513513513No Hit
CAGGATACACCATGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA11.3513513513513513No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA11.3513513513513513No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA11.3513513513513513No Hit
CAAGAACGACATCAAACACACCAGCACACCAGCTAGCAAGAGACCCAAAA11.3513513513513513No Hit
CCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTA11.3513513513513513No Hit
GCCAGACACCGACCAAAACAAAACCCAACCCCAACAAAAACACTCCAAAA11.3513513513513513No Hit
TAAACGCAAAGAATTAAAAATAAATGAAAATAGCAATCTTCAACAATAAG11.3513513513513513No Hit
AAATGGAAATCCGAATACATGAAACAAACTTCCAAATGTGTGCATATTGC11.3513513513513513No Hit
CATATATACTGATGAATGCACACTTTCCCAATATAGACTTCCTCCACTAA11.3513513513513513No Hit
GCAGCACCACACAACCACACACACCCACAACGCCCAACACACAACCCACC11.3513513513513513No Hit
CAATGGTGCTGGCTAGCACTACGGCAAAGGATATGGAACAGATGGCTGGA11.3513513513513513No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA11.3513513513513513No Hit
AACAAATGATAGTAGAGCATGCGCGAACCGCACAGAAAGAAGATGCCTAA11.3513513513513513No Hit
CATAAGTATGTCCTGGAATATAAGGTAATGGTGAAATTTCTCCAACTATT11.3513513513513513No Hit
GACATCAAAAAACCTAAAAAGTAAGAGGAAACAACATTGGACTCGATATC11.3513513513513513No Hit
ATCCAAAATGAACTAAATTTCAAATTCAAACACAACAACAAATAAACATC11.3513513513513513No Hit
CAACCAAACATACTGAACAATCATAAGCAAAATTCAAAAATCAGCCGAAA11.3513513513513513No Hit
ATAATCAAATGTCTGCTGGAAATGTGTATCTCCTTGCCTTCATCCACATC11.3513513513513513No Hit
GTCTCTGTGTTTGTCGTCCACTTTCCCTTTTCTGAGTACTGTGCTGTCTC11.3513513513513513No Hit
ATATACAACACGATCGCAACAGACACCACACAAGCAGCTAATCGACTAGT11.3513513513513513No Hit
CCACAGCAGACACCACCCCAGAACAGCCACCAACGCCAACAACACACAAA11.3513513513513513No Hit
ATCAGTAACAATGCTATACTCACGTTTTCGTATCATTAACAAAACAACGA11.3513513513513513No Hit
AGATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTG11.3513513513513513No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT11.3513513513513513No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT11.3513513513513513No Hit
CAATTAGCACATTACAATTCTCTCCTTTTTCAAGATTGCTCAGTTCATTG11.3513513513513513No Hit
GTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCAGCACATTAG11.3513513513513513No Hit
GAAATCTGATGTTCAACTTGAAATAATTACATCATGGACTCAGCCAATCA11.3513513513513513No Hit
GGGTATCACAAACCAAACATCCAATACAAAAAGACACCGCGGATCATCAC11.3513513513513513No Hit
TAAAAGCACTGGAGCTAGTAAAAGTCCCAAAATATATCATTGCATGTACC11.3513513513513513No Hit
CTTTTAATTTCTGCACTTGAGGTAGAGAATCACTCACATACGCATCAAAC11.3513513513513513No Hit
AAGCAGTAGTTGTAAGTCTTGAATAAAACTTATTTCTGTAACTTATACAC11.3513513513513513No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA11.3513513513513513No Hit
TAATATAACCAATCAAATACCAAGTGCAAAATTATAAAACATTAGATACA11.3513513513513513No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT11.3513513513513513No Hit
CTGGGGGCCCAACCGCCCGCCACCTGAGCGAACCCCCCCGACCGCCCCAG11.3513513513513513No Hit
GCCCGAGGGGTGGGGGCGGGGGCGTCGTGGGGGGGGGGGGTGGCGGGGGG11.3513513513513513No Hit
CTTTAGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTATTAG11.3513513513513513No Hit
CTTCAGAGCAAGAGAGAGAAAGAACAAGAAACAACAACCAAAAACCAAAA11.3513513513513513No Hit
AAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACCTGTC11.3513513513513513No Hit
CCATATGGCCATAATCAAAAAGAACACATCAGGAAGGCAAGATAAGAACC11.3513513513513513No Hit
GATCTATTCAACTGTCGCCAGTACATTGGTACTGGTAGTCTCCCTGGGGG11.3513513513513513No Hit
CAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTAGGCTGAATCTA11.3513513513513513No Hit
TTATTAAATTGTCTGCTGTAATAGGGTATATCATTGACATCATACACAAA11.3513513513513513No Hit
CACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGACT11.3513513513513513No Hit
CTTCTGCTACATCTCAACAACCACAAGAATACCCCTATCGCTTGAGTTCA11.3513513513513513No Hit
GCCATTATGAGTGCGGGGTACTTCACTTGCCTTCATGATGTGTACTCTTT11.3513513513513513No Hit
TACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAAT11.3513513513513513No Hit
CATCCATAAAGCAATGGAACAGGAACAGGATACACCATTGACACAGTAAA11.3513513513513513No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC11.3513513513513513No Hit
TCGTAACACCCAACCACACTCTCAAAATATACACACAAACCCAACCACAA11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)