FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_F2_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_F2_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC21.7543859649122806No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCCTGTCTCTTATA21.7543859649122806No Hit
ACATAAAACCAAAACGACCAAACCAAAAAAACAACAGAAAACAAAAACAA10.8771929824561403No Hit
GATCTCCCCCAGAGCACCTGGGTGGCTCAGCTGGTTGGACACAAGTCATG10.8771929824561403No Hit
GTGCAAAGCCACCAATACACAGAAATATACCGACTCCGCAAACTCCCCAT10.8771929824561403No Hit
ACCACGTTACGGCCAGAGCAGATACCATCACTCGGTCTGATCCAGCATCG10.8771929824561403No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTGCCAT10.8771929824561403No Hit
CCATCAACCACGACATACAGAGCGCGGGGTGCGTCGCGTGCCTTCCGGAT10.8771929824561403No Hit
CTAGCCATATGTCACAGAAATACAAATATATAAACAGACAACTCTTGACA10.8771929824561403No Hit
ATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTT10.8771929824561403No Hit
TTTCAATGATGCTTAATGATGGGCCATAACACTACTCTTCTATGCCCAAA10.8771929824561403No Hit
CATTGCCATCATCCACTTCATTCTGAGTGCGTGGTTCTTCTCTGGCCTTC10.8771929824561403No Hit
GATTGTAACTGTGTCAAAGTGAGATTGTTTTTGTCTCTTTTTTTCAAAGT10.8771929824561403No Hit
GCAAAAAAAATAAATGAACACAGCAAACTTGCAAAAGGAAAGAAAGCAAA10.8771929824561403No Hit
GTTTTAAAAACCAAAGGGAAATCAACAGAACTTCAACTAGCGCCAAACCT10.8771929824561403No Hit
TAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACCTGTCTCTTAT10.8771929824561403No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA10.8771929824561403No Hit
ACTCACCCCCCACACCCCCCCACCCCACCCCCCCCAACCCCCCCCCCCCC10.8771929824561403No Hit
GACTTACACAACAACGAAACCAACCACCAGCAAACACTCAAGATCCACAT10.8771929824561403No Hit
CTAATCGATGGCCATCCGAATTCTTTTGGTCTCTGTCTGGCTGTCAGTAA10.8771929824561403No Hit
CTTTTAGACTAGGAAGCAAGGGAAGAACAGTGGTTATGTATTTGGATGTA10.8771929824561403No Hit
TCATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCT10.8771929824561403No Hit
TTACAAATCCACCATGTAACTTAAAAATGAAAAACAATCAATAGATATCA10.8771929824561403No Hit
GCCAATTCAGATGCTTTCTCAAGACTAAGTTAATAAGGCTCCTGTGTTTT10.8771929824561403No Hit
AAACAACCCCCCAACACACCAACACCACCCCACCCACAACCCCCCCCCCC10.8771929824561403No Hit
CAATTACAACAAGGCAACCAATCGACTTACAGTTCTTGGAAAGGATGCAG10.8771929824561403No Hit
GGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCAC10.8771929824561403No Hit
ATGAAAGAGACAAGATTTTGTTTCATTACCAACACAACGTCCACTCAGTA10.8771929824561403No Hit
AATCAATAACATTCAGACATTATACTCTAGCAAAACTCAAGAGAACTTTT10.8771929824561403No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT10.8771929824561403No Hit
TCCAAAAAATCATCACAGATTTATAAAAGAATACCACAGGTTTGGAAAAT10.8771929824561403No Hit
TCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGC10.8771929824561403No Hit
CTTCCAGCCTTGCCTTTGTCCCATCCTGACTTACTGTGTTCTTCAGTACT10.8771929824561403No Hit
CGTTAGTTATGCACAAGAGGGAAAGTCCATGTTCATTCAACTCTGCAATC10.8771929824561403No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA10.8771929824561403No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT10.8771929824561403No Hit
GAACAAACACGCAATCATAAAAAGGTAAACAAAGACAAGGCAACAGTAGA10.8771929824561403No Hit
CTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGAC10.8771929824561403No Hit
ATTCCAGAGAGGAATGAACAAGTACAAACCGTCGGGAGCAAAACAAACGA10.8771929824561403No Hit
ATCCAGAACAACCCACAAAACGAGAGGAATCTCCACACCTCACACGAAAA10.8771929824561403No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC10.8771929824561403No Hit
ACATCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT10.8771929824561403No Hit
AGGCCAGATAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAC10.8771929824561403No Hit
CTGTGGACCATATGGCCAAAAACAAAAACTACACATCACGAAGGCAAGAG10.8771929824561403No Hit
GTACAGTTCATTACCCTAAGGTATATAAAACTTAATTCGAAAAGCTCGAA10.8771929824561403No Hit
CACACAAAAACACACACAAACACACACACACCCACACAAACACAAACAAA10.8771929824561403No Hit
CCTTTTGCAAGAAACCACAGTACAATCATGCTTAAAGCACGGCCATAAAA10.8771929824561403No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA10.8771929824561403No Hit
GAACACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC10.8771929824561403No Hit
ACAAAAAACAAAGAGTAACAAATGGACAGCAACAAAAACAACAAATAATG10.8771929824561403No Hit
ACCATGAAACCGGAAAGACCTGAAACCACAACTCGGTCTGAACCAGAATC10.8771929824561403No Hit
CTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCT10.8771929824561403No Hit
AGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGA10.8771929824561403No Hit
ATTTGTCACTGTGTGTGTTTTTCTTCTGTAACTTATAAAAATAACACGCT10.8771929824561403No Hit
AGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTCTG10.8771929824561403No Hit
CTGCGGGGGTGGTAGGGTCAGACGCGGCCGGTGGCAGCGGCAGTTGGGGT10.8771929824561403No Hit
GCATTAAGCAACAAAGAACAGACCAAACATGCAAAACGAGAGAACGCAAA10.8771929824561403No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT10.8771929824561403No Hit
GCTAATGCTCATTGGGCCAGGGGACGTAGTGTTGGTAATGAATCGAAAAC10.8771929824561403No Hit
CTGCAGCCCCCCCACCCCAACCCAACACCCCCAAACACCCCCCCCCACCA10.8771929824561403No Hit
GTACTACTTGAATAAGCCCATATGGACCACAGACGTCTTAGTGAGTATCT10.8771929824561403No Hit
GTCGGGAGGGAGTGGATGTTGGCACGGGGATTCCCTGTTCCTGCCGACAA10.8771929824561403No Hit
CATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTC10.8771929824561403No Hit
ATTCTACGCCATATCTCTTGTATTCATTGCCCAAAATGAGAAATCCTCTC10.8771929824561403No Hit
AGTTTGGAGTGGGGAATGGTGACAGATAATGCTGGAAAGGTAGACTGGAA10.8771929824561403No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC10.8771929824561403No Hit
AAAGGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGG10.8771929824561403No Hit
ATATAAAACACTACACAGCACTAAAACCCAAACACAAACCCAACAACCAA10.8771929824561403No Hit
AACCACCAACCAAACAAAAACAACACACACAAACACCAACAACAAACCAC10.8771929824561403No Hit
TCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGT10.8771929824561403No Hit
TTACCAACACTACGTACCCTTGCCCAATAAGCACATTAGCGTTCTCTCCT10.8771929824561403No Hit
GAGCAAACATCCAAAACGAGACCACCCAAAAGAGCGCAATCGCCCACGCA10.8771929824561403No Hit
CACTACGTCCCCTTTCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA10.8771929824561403No Hit
TGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGCTGTCTCTT10.8771929824561403No Hit
CCAAACCACAAAAAGAACATGCAAAGGAAGCACGTGCAAAAACTCAAAAA10.8771929824561403No Hit
CTATTGTCAGCTGTAAATGGGTATCTCAATGCCATCAACCACTTCATACA10.8771929824561403No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT10.8771929824561403No Hit
TCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTC10.8771929824561403No Hit
GCAATCCATTACACAAAACAAAACGCTGGGAAACGAACAAATACCATAAC10.8771929824561403No Hit
CCCAACTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG10.8771929824561403No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTGTCTCTTATA10.8771929824561403No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG10.8771929824561403No Hit
GATGGGAACACCTCTCGCCCCCCCCGCCCCCCCCACCAAGGGCCCCACAA10.8771929824561403No Hit
ACATTACCCAACACACCAAGAGCAAGATAGCTCACAACAGCCCACTCGCC10.8771929824561403No Hit
GTCAGCAACCAACCCTCCCGGTCACCACCCCGGACCACCACTCCCGCCCC10.8771929824561403No Hit
AAAGTGGACCATAAGGCAATAATCAAAAATTACACATAAGGAAGTAAAGA10.8771929824561403No Hit
AATTAGCACAAAAGCGATCTCTCCTTTACCAAGAAACCACAGTTCATACA10.8771929824561403No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC10.8771929824561403No Hit
GACTTACACAATAAGATTGTGTATGTGTGTCTTTTACAGTTATTGATTCA10.8771929824561403No Hit
CTTTTAATCTGTAATTTGGTCTCAAAAATCAACAATACTTTCAATAAAAA10.8771929824561403No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC10.8771929824561403No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATTATTCT10.8771929824561403No Hit
CGAGATACACACAAAGACCACTCACGACCAAAACACACACATATACACAT10.8771929824561403No Hit
ACCCAGCAAAAAAAATCAATCAACAAACCAATCAATGAAAAAACTAGAAC10.8771929824561403No Hit
GCTGTAATAGTGTATCACATTGACATCATCAACTACATACTTAGTCATTG10.8771929824561403No Hit
TCAGAATAACTTAGCTAGATTTATTAATTTCACAAAAAATAATACACAAC10.8771929824561403No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCCTGTCTCTTA10.8771929824561403No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG10.8771929824561403No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA10.8771929824561403No Hit
CACACCCCCCCACACCCCCAGACCGATACCCACCACCCCAACCACGCCGC10.8771929824561403No Hit
GGAGTCAGATGGGGCTCCGTTGGGAGGTCGCCTGAGTGGGTGTCTTGGGC10.8771929824561403No Hit
ACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCA10.8771929824561403No Hit
CACAACCTCCCCAACCCCCCATACCACCAGCGCGGTCTCACCCTCTCCCC10.8771929824561403No Hit
TAATGGTTCCCTGTCTTTCATTTAGGTCTTTCATCCATCTGTCTCTTATA10.8771929824561403No Hit
CTAAGACCACAGAGGACCAAAACGCCAAAAACAAAAAGAACACAACCACT10.8771929824561403No Hit
CCTTCAAGAACATGATAAACAAAACTAAAGTCAACAAACAGAATTAACAC10.8771929824561403No Hit
GAGTAAAAGGGTATTTCTTCAGATCAGATGCAGGGAAGAGTGACTGTCTC10.8771929824561403No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGCTGTCTCTTATACA10.8771929824561403No Hit
CTACTAGCAGCTTCTCGCCGTATCAGCACGGGTGACTCCCCTCACCTCGA10.8771929824561403No Hit
GACGTAGTCTAGGAAAAGAAAACAAAAACCGACACAAGCAAACAAACACA10.8771929824561403No Hit
ATGCACAGAACATTTATGCAAAATCCAGAACCATATATCAACCCCTGACT10.8771929824561403No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA10.8771929824561403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)