FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_E6_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_E6_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG24.545454545454546No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC24.545454545454546No Hit
GGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCAC12.272727272727273No Hit
CAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCA12.272727272727273No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTCTCATTGCC12.272727272727273No Hit
CTTCCACACTTATATTTAGAATCAGTCACACAAAAAAAAAAACAACATCA12.272727272727273No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA12.272727272727273No Hit
AAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAACTACC12.272727272727273No Hit
GGCAGAAGCCTTCCACTTATTCAAGACCGCCTTTGACTTCTTTTATAACA12.272727272727273No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC12.272727272727273No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG12.272727272727273No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCTGTCTC12.272727272727273No Hit
AAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGCTG12.272727272727273No Hit
CTCTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATA12.272727272727273No Hit
GATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCT12.272727272727273No Hit
AGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATTATTCTCTTGT12.272727272727273No Hit
CAACAAGTACAGTTCATTACCCTAAGGTATATAAAACCTGTCTCTTATAC12.272727272727273No Hit
GTTGAAAGCACGAAGACCATATTACAAAATACATAAGTAATGAAAATCAT12.272727272727273No Hit
AAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAACGACC12.272727272727273No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCTGT12.272727272727273No Hit
ACACTCTATTCCATTTTGTTCCTGCAAACAATTTACCAACCCGTTTCTCC12.272727272727273No Hit
AAATAGTCTCGTGTCAGATGGAATTTGATAGCGAAAATCCAACAACGACA12.272727272727273No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT12.272727272727273No Hit
CAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG12.272727272727273No Hit
GTCCTGCACCGCGGGTACAACTGAGGGAGCCAGTCACAGTACAAATACCT12.272727272727273No Hit
GCATACAGCCCGATGTGGGACTCGATCCCAGGACCCTGAGATCATGACCT12.272727272727273No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCCTGTCTCTTATA12.272727272727273No Hit
AAAGCATCCCTAGCACAAGCCAAATAACCCATAGGTGGAAACAGCCAACC12.272727272727273No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT12.272727272727273No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA12.272727272727273No Hit
GAAGGAGGGAAAAGGGGAGTGAGGGAAAGGAGAGATTATAACTTTTTCTT12.272727272727273No Hit
GGTACACCTGGGTGGCTAAGTCAGGGTTAAGGATCTGCCTTGGGCTCTAG12.272727272727273No Hit
GTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATT12.272727272727273No Hit
GGCAATGAGATACCCAATTACAGCAGACAAGAGAATCTGTCTCTTATACA12.272727272727273No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT12.272727272727273No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG12.272727272727273No Hit
TTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTCTCTCCATATCT12.272727272727273No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC12.272727272727273No Hit
GGGATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCTCCAGTAT12.272727272727273No Hit
CAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGCTGTC12.272727272727273No Hit
GTATTAGTCTAATACGATAGGATTAACCTAAGGTATACACCTGTCTCTTA12.272727272727273No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)