FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_E4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_E4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTAGTGAGTATCTCGCTATTGCGGGACTGCGACATTAGATATCTCA13.8461538461538463No Hit
GATAAGCAACATCCATGGACTCCGAAGACTGTTCACACTAACCAGCAAAC13.8461538461538463No Hit
ATACACTGTCGCTATCAATCTATCGCGCAAATCTACAATAAACCACATTA13.8461538461538463No Hit
GCACTGCAGCACGGAACCCTCACCCCGACACAACACCGACACACCCACCG13.8461538461538463No Hit
CTATTGGGAAGAATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT13.8461538461538463No Hit
GACAATGGATTGGGACAAGCCCCATTCCTAGATCTGCTCCGCAGAGATCA13.8461538461538463No Hit
CCATCACTCGGTCTCAACCACCATCGTTAGATTTGCTCCAGAGGGTTTCT13.8461538461538463No Hit
GGCAAAGGCTATGGAACAGAAGGCGGGATCGCGTGTCCAGGCAGCGGAGG13.8461538461538463No Hit
GTCAGGCTCACAAAAACAAAACAAAACTCCAAAAAACAATACAAAAAAAA13.8461538461538463No Hit
GACTAGGCAGAAGGTACATGCAATGAGAAATATTGGGACTCATCCTAGCT13.8461538461538463No Hit
ATATGGAGAGAATAAAAGAACAGAGAGATCTAATGTCGCACTCCCGCACA13.8461538461538463No Hit
GTATAGTTTAACTGCTCTATCCATGTTGTTAGGGTCCCCATTCCAATGTA13.8461538461538463No Hit
TCCTTGTTGATTCCAGCTTTACACCATCTATTTCTTCTCTGTTTAATTTT13.8461538461538463No Hit
TACCAGAAGCGAATGGGAGTGCAGATGCAGCGATACAACACAACCTCTCG13.8461538461538463No Hit
ATATACCAAAAGAAAAACAGTATCTTCGAATGATAATTTTTCTCCCCGAT13.8461538461538463No Hit
CCAAAAAACTCCAGATCCACCATACAGCCAAGCAAAATGAAAACTATACA13.8461538461538463No Hit
ACATAATGGACTCCAACACAATGTCAAGCAATCAGGAACACTCTTACCTA13.8461538461538463No Hit
AAAAAAGCAATTAAAGATCCAAAAAGAACAAAAATTTTAGACAATGAATC13.8461538461538463No Hit
GCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAA13.8461538461538463No Hit
CAAGTTAAATTCATTATTTTTTCCGTCTGAGTTCTTCAATGGTGGAACAT13.8461538461538463No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC13.8461538461538463No Hit
AATAATGGAAAAGTCACAGCCACATGTGAACACATACATAAATCAAAGCA13.8461538461538463No Hit
TCCAAAGACAAATGCGAAAACGATTTACACACAGTTCATTGAGACATAAG13.8461538461538463No Hit
CTCATAAGTATGTCCTTGAAGAGAAGGTAATGGTGAAATTTCTCCAACTA13.8461538461538463No Hit
GTTGTAAGGCTTGCATAAAAGTTATTTGTTCGAAAATATACTCTGTCTCT13.8461538461538463No Hit
CACCAGGCGATCATGGAAAAGAACATAGAACTGAAATCGAACTTCAGTGT13.8461538461538463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)