FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_D9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_D9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTGACCCTGACAGCCAATAAGGAGTCCCAGGTATAGCTCTCTCTCTT12.941176470588235No Hit
CTGTGGACCATATGGCCATAATAAAAAAGTACACATCAGGAAGGCAAGAG12.941176470588235No Hit
CTACAAAAATTAATTAAAAAAATACACAAACAAAAAAAAATATAATGCCC12.941176470588235No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAACTGTC12.941176470588235No Hit
AAAAAAACTGCACAAATTTTACCACTACTATGATGAAAAACATCAAACAA12.941176470588235No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC12.941176470588235No Hit
ATCCAGGGAAGCTTATTGACACGGCTTCCTCAACGTGGCAAACGGCAAGG12.941176470588235No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATCATCCACTTCATTCTGA12.941176470588235No Hit
GGTTATACCATCTCTCTCTCAGAACAAGCAAACTTACTTAACCTCAATAG12.941176470588235No Hit
AACAAACAAAAAGCAAACAAAACAAAAAACAGTACAAGCAAATGATTCAT12.941176470588235No Hit
GAATAATCCCAAGATTAAACATCAACTCTATAATCAAAAAAACCACAATC12.941176470588235No Hit
CAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTG12.941176470588235No Hit
GTGATGGTAATGAAACCAAAACGGCACTCTATCATAATTAGAGACAGAAA12.941176470588235No Hit
ACAAACATAAGTAACATCAGACAATGCACATACAACCTTACCAAAAACTG12.941176470588235No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT12.941176470588235No Hit
ATAAACATTCCCGATGTCATTTCATAACACAAACACATAAACCAATACAA12.941176470588235No Hit
ACTGTGGACCCTATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA12.941176470588235No Hit
GATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCT12.941176470588235No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT12.941176470588235No Hit
GAGCAAAAGCAGGGAGGGGGGCACAGGGGAAGGGGCAGAGGGAGAACAGG12.941176470588235No Hit
AAAATCTTCGAAATATAAATCAGTCAGAGACACATAATATTTGTATCAGA12.941176470588235No Hit
GAATAAACAAACACGATTAATAAATATTCGAAAACTAATTGATGAACAAC12.941176470588235No Hit
CAATAATGTCCATTAATCACTTTTCTCCTTAAAATTGTAAACTAATTGAC12.941176470588235No Hit
AATAATTGAAAGTAATCAAAAGAAAACACTAAACAACCATAACAATCCAC12.941176470588235No Hit
GTCAACAGAGAATATATCAAATTGTGCAAAAAAAACAAAAGATATATCAA12.941176470588235No Hit
GTCCTCCAAAATGCTGTGAAGAACAACAAGACATCAATAAGAAAAGATAG12.941176470588235No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG12.941176470588235No Hit
ATAATAATATCTCCAAGAATTAATCATAAATGAACACAGCTATATCATTC12.941176470588235No Hit
GTGTGGGTGGCCCAGGCGCCGGAGCGGGCGCCGGCGCGCGCCGGACGGGA12.941176470588235No Hit
TAAGAATGTACAATATAAAACATAAAAATCAAAATATCAAATAAACAAAG12.941176470588235No Hit
ATTCAATATCGAGAGAAGAAAACAACTGAGAGAACTAAAGCCTAAGACCC12.941176470588235No Hit
GCAAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCCCCACTGA12.941176470588235No Hit
GTAAATGTCTGAAGTTCTACTTACGCCATGCCGATACCTCCAATTTAATC12.941176470588235No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)