FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA25.263157894736842No Hit
TACAAGGGGACGTAAAGATTGTAATAAAACAAAAACCTGACTCTATCATA12.631578947368421No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG12.631578947368421No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC12.631578947368421No Hit
ATGCGAGAGTCACGTTTTCGTTTCATGAACAAAACAAAGTCCCATTGCCC12.631578947368421No Hit
CTCAATCAACTCAACTTACCCGATTAAGAGTATTTGATATTTCTGTATAC12.631578947368421No Hit
GAAAAAGAACAAGATAAAACATCGAAAAAATAAAAAAGACAAATTTTTCA12.631578947368421No Hit
AGCAATACTCCAGATTTCAAATAATTCACAAAACAAAAAACTAACTAAAA12.631578947368421No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA12.631578947368421No Hit
TTTAAACAATTCCAAAAAATAACTCAGGACATCACCCCAACTTAATTATA12.631578947368421No Hit
TTTCATAACCAACAAAACTTCCACTTGCCCAATTACCAAAATTCACAAAA12.631578947368421No Hit
CAAGAGAGTTTCTCATTGAACATTCCTCACACGAATCATGTCCATTATTC12.631578947368421No Hit
CTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCC12.631578947368421No Hit
AAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAG12.631578947368421No Hit
ACATTACTGGCTCCATTGTGACTCCTTGACCTGTTCCATGTCTCAATGGC12.631578947368421No Hit
CAAAGGGACCAGGTATTTATATGAAGAACAGATGGTTTGAACAGAGACTG12.631578947368421No Hit
CTATAACTCCATGAAAAATCAAAAGCAAATACCTACACTCAGACTAAAAC12.631578947368421No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC12.631578947368421No Hit
TGTGTGGGTTGATTTCTGGACTCGGTTCTATTCTCCAGCCCTTGTTCTGT12.631578947368421No Hit
TGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGT12.631578947368421No Hit
CCTTAAGAAATAATTCAAAGATCAAGCATGCATAAAGAATTGAAATATTA12.631578947368421No Hit
CAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGAATTACAGTTC12.631578947368421No Hit
TAACATGGTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCT12.631578947368421No Hit
ACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACCTGTCTCTTATAC12.631578947368421No Hit
AACTAAGAACTCTAACACAATTAGCACATTATATTAATCTAATAAAACAA12.631578947368421No Hit
GTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATT12.631578947368421No Hit
GAATATGAGGCCCATGCAACTAGCAAGTGCACAATATGAATAGCATATTG12.631578947368421No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA12.631578947368421No Hit
AAATCATGCAAAATAACATAGAAAAGAAATCTTAAATAAGAGTAATATTT12.631578947368421No Hit
TGAAAAAATGATGCAGTTGCAACTACACATTCCTGGATTACCAATAGGAA12.631578947368421No Hit
ATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGAT12.631578947368421No Hit
GTTAAAATATATTCAATACGGAGACAATAAAAGAACTGAAAGATCAAAAG12.631578947368421No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA12.631578947368421No Hit
CCAACAATTTTACAAACAACGAAACACAAATCTAAACAAATATTGAAACT12.631578947368421No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC12.631578947368421No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA12.631578947368421No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)