FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_D1_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_D1_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6410
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG370.5772230889235569No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC300.46801872074883No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA230.35881435257410293No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA190.296411856474259No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT190.296411856474259No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT180.28081123244929795No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC160.24960998439937598No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC150.234009360374415No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT140.21840873634945399No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA130.20280811232449297No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA130.20280811232449297No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC130.20280811232449297No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA130.20280811232449297No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA120.187207488299532No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA110.17160686427457097No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA110.17160686427457097No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG100.15600624024961No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT100.15600624024961No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA100.15600624024961No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA100.15600624024961No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC100.15600624024961No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC90.14040561622464898No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA90.14040561622464898No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA90.14040561622464898No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA90.14040561622464898No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC90.14040561622464898No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT80.12480499219968799No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA80.12480499219968799No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG80.12480499219968799No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT80.12480499219968799No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC80.12480499219968799No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA80.12480499219968799No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA70.10920436817472699No Hit
ACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGG70.10920436817472699No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC70.10920436817472699No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG70.10920436817472699No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG70.10920436817472699No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC70.10920436817472699No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG70.10920436817472699No Hit
GTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCA70.10920436817472699No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA70.10920436817472699No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA70.10920436817472699No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT70.10920436817472699No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC70.10920436817472699No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA70.10920436817472699No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC70.10920436817472699No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC70.10920436817472699No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA70.10920436817472699No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT70.10920436817472699No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA70.10920436817472699No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT70.10920436817472699No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG70.10920436817472699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)