FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_D1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_D1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAATCAAAGTAAAAACAAATAAACAAAAACAAATTCCACAAAATAA15.88235294117647No Hit
GCGAGACACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTT15.88235294117647No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCTGTCTCTTATAC15.88235294117647No Hit
AAAAAAAACTCAACAACATATTTGCCAACAACTATAACAACATTAAAAGC15.88235294117647No Hit
ATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGA15.88235294117647No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCTG15.88235294117647No Hit
GGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACT15.88235294117647No Hit
GCGGGGGCCCCGGGCGCGGGCGCGGGCTCGCGGCGGGGGGGCGCGCGGCG15.88235294117647No Hit
GATCAAATCATATTTCATCCACACAACCTTCAGTAAAGAAAAACCTTAAG15.88235294117647No Hit
CGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATGGATCT15.88235294117647No Hit
CATAAAACACACAAAGAAAACAAACACCAAACTGGAAAGAAAAATAAAAC15.88235294117647No Hit
AATCAGGAACAAAAGACAATAAACAAAAACTAAAAATCAAGCAGTCAAGA15.88235294117647No Hit
GCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCACTGTCTCTTATAC15.88235294117647No Hit
TACAAAAACAGAATCAAGAACTCTGTACTTACATTCAATAACACATACCA15.88235294117647No Hit
CTGATGATCAACATGAAGACACAAAATGCGATAACACTGATTTACTGATC15.88235294117647No Hit
CTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCTGTCTCTTA15.88235294117647No Hit
GCCATAATCAAAAAGTACACATCAGGAAGACAAGCTGTCTCTTATACACA15.88235294117647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)