FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_A4_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_A4_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAAATGTGTGCATATTGCAGCAAACTTGTTAGTTTCGATTTTCGGA12.564102564102564No Hit
GGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGAGAATAGT12.564102564102564No Hit
AAATAAAATTCAATTAGTCAGAAAATAAAAGATACGTATATAATAAATAA12.564102564102564No Hit
ATGGAATGGGGACCCGAACAACAAGGATAGAACAGTTAAACTATACAAGA12.564102564102564No Hit
GGTGAAATTTCTCCAACTATTGCTCCATCCTCAGTGAAAGCCCTTAGTAG12.564102564102564No Hit
TTCCAATGGAACCATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCC12.564102564102564No Hit
TTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGG12.564102564102564No Hit
CACTGAAATAGTTGTTGCAAATCTAATATAAGCTATCAAAACCAGACTCA12.564102564102564No Hit
ATGAAGGACGGACATCCGACATGCGAACAGAAGTTATAAGCTGTCTCTTA12.564102564102564No Hit
AGATTGCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTC12.564102564102564No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGAAATCTTGCAAAAGGA12.564102564102564No Hit
GAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGT12.564102564102564No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT12.564102564102564No Hit
GGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATG12.564102564102564No Hit
ACGTTCAATTGTGACAATGATGTGTCACTAACCTATTCAACTGGTGCACT12.564102564102564No Hit
CGCTCAGATCCAGCATCGTTTGTTATGCTCCAGAGGGTTTGTCCTAGTTC12.564102564102564No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGACTGTCTCT12.564102564102564No Hit
AAATAAAAATAAGTGATTAAAACTAAACGATAAAAAATAAAATAGAAAAA12.564102564102564No Hit
GTGCGGGGTACTTCTCTTGCCTTCCTGATGTGTACTTTTTGCTGTCTCGT12.564102564102564No Hit
GTAGAAATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGTCCCT12.564102564102564No Hit
CTGCATCCGCACTCCCATTCGATACTGTTAGGACTGCAAAAATTAAAGAA12.564102564102564No Hit
TATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCG12.564102564102564No Hit
ATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGA12.564102564102564No Hit
CTCAAATAGAGAACAGTAATAGTTCATACTACCAAAATCAAACATCACAT12.564102564102564No Hit
GCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT12.564102564102564No Hit
CTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAA12.564102564102564No Hit
GGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCC12.564102564102564No Hit
AAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGAG12.564102564102564No Hit
GGAAAGAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACC12.564102564102564No Hit
GTATGCAAGAGTCCCGTTTTAGTGTCATTACCAACACTACGACCCCATTC12.564102564102564No Hit
ATTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAAGC12.564102564102564No Hit
ATAAAACAATTACCTACTATAACACAACAAACTTATAACCATAACAAACA12.564102564102564No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG12.564102564102564No Hit
CTACGTCCCCTTGCCCAATTAGCACATAAGCCTTCTCTCCTTTTGAAAGA12.564102564102564No Hit
AGCTTCTTGTATAGTTTAACTGCTCTATCCATGTTCTGTCTCTTATACAC12.564102564102564No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGCTGT12.564102564102564No Hit
AGCGGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTC12.564102564102564No Hit
CTTCAGTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTT12.564102564102564No Hit
ACCAGAAGCGAATGGGAGTGCAGATGCAGCGATTCAAGTGATCCTCTCGT12.564102564102564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)