FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_A4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n02_CIVIR_Ferret_Flu_Tissue_P1_A4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATG17.6923076923076925No Hit
ATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGCGTCT17.6923076923076925No Hit
ACTGCGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA17.6923076923076925No Hit
TTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTC17.6923076923076925No Hit
AATGGGAACACAGTACTGCTCTACTTTGCGCGCCTTTGAACATGGGACTT17.6923076923076925No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA17.6923076923076925No Hit
GTGGGGCGGGGGCCGGTGGCCTGGGGGGGGGGGCGGGTGGGGGGCGGGGG17.6923076923076925No Hit
CACAACAAACCAATTCCAAAATAGCAAAAAACCCTTCACTACAAAACCAA17.6923076923076925No Hit
TTTCTGAGTATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTT17.6923076923076925No Hit
ATCCCAGGGTCCTGCGATCAAGTCCCACACCAGGCTCCCTGCTCAGTGGG17.6923076923076925No Hit
CAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACCGTGTT17.6923076923076925No Hit
GGCACGGTGAGCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGA17.6923076923076925No Hit
TTTCTCCAACTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAG17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)