FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H7_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H7_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3749
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC190.5068018138170179No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT140.37343291544411844No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC120.3200853560949587No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG110.29341157642037874No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT110.29341157642037874No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG100.2667377967457989No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA90.240064017071219No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC90.240064017071219No Hit
TCCAAATACATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATT90.240064017071219No Hit
CCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTTCGACTAA90.240064017071219No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA80.2133902373966391No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG80.2133902373966391No Hit
CTCATATGCATTTGACAAAAGAACTACTGATGGAGTGTTATTATGTCTTA70.18671645772205922No Hit
ATATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGA70.18671645772205922No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG70.18671645772205922No Hit
GTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTT70.18671645772205922No Hit
GTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTTTGGTTG60.16004267804747935No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC60.16004267804747935No Hit
CTCCAGAACCCATCCACATGCCGGTTCTCTGAGTTCCAGAATTTCCCCAG60.16004267804747935No Hit
GGGTAGAGGTAGAGGGAGAAGCAGGCTCCCTGCTGAGCAGAAAGCCCAAC60.16004267804747935No Hit
GTCTACGGATTCATGTTTGAAATGTGCAATATTGGCAATCAAGACTTACC60.16004267804747935No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA50.13336889837289945No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC50.13336889837289945No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.13336889837289945No Hit
TTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAG50.13336889837289945No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA50.13336889837289945No Hit
GGTGATTATTGTTCTTTCTCCCCAAAGTAGTCTTTCCTGATGTCTGACTG50.13336889837289945No Hit
ATTTTTAACAGGATCAGCACTGGAGGAACGCAACAATGCATTTTATTAAG50.13336889837289945No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA50.13336889837289945No Hit
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA50.13336889837289945No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT50.13336889837289945No Hit
CTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGT40.10669511869831955No Hit
TGTTTGAAATGTGCAATATTGGCAATCAAGACTTACCTCAGCATTTGCTG40.10669511869831955No Hit
ATTCTATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGC40.10669511869831955No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA40.10669511869831955No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA40.10669511869831955No Hit
CCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTGGCTGGGAATGG40.10669511869831955No Hit
ACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGAGTTAC40.10669511869831955No Hit
GTCTTGATTGCCAATATTGCACATTTCAAACATGAATCCGTAGACCATTT40.10669511869831955No Hit
ATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGCCTGTG40.10669511869831955No Hit
CAACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGA40.10669511869831955No Hit
GTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCTAATTA40.10669511869831955No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT40.10669511869831955No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG40.10669511869831955No Hit
CAATAATGGTCAACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACA40.10669511869831955No Hit
CTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACC40.10669511869831955No Hit
TATCAAAACTTATCAGCTGTAGATAAAGCTGTCTAGAGGAAATTGTATAG40.10669511869831955No Hit
GTATTAGGGAACAGAACGAGTTACCAGGAAGCTCCCATACGGAATTTAGG40.10669511869831955No Hit
CATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTTCG40.10669511869831955No Hit
CCTAAATTCCGTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATAC40.10669511869831955No Hit
GGCAAATGCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGA40.10669511869831955No Hit
GGGTTGGTTATTGACTGAAACTGGGGATGAGGTAATGCTCTTTGTTGACT40.10669511869831955No Hit
CAATTATACCTAACCACAGACTGGAATTTCCTTAATAAAATGCATTGTTG40.10669511869831955No Hit
AATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAA40.10669511869831955No Hit
GTTCCAGAATTTCCCCAGCAGGAGGACTGGTTTGCTAATCTTGTTTTTTT40.10669511869831955No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA40.10669511869831955No Hit
CTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGA40.10669511869831955No Hit
CTTTTAGAGACAGATAATTAGAGCATTGGTGTCTAGCCCTTTTTTTCAAA40.10669511869831955No Hit
GCATTTGACAAAAGAACTACTGATGGAGTGTTATTATGTCTTAGGTGGAG40.10669511869831955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)