FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H5_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H5_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11930
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG380.3185247275775356No Hit
GCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGC250.20955574182732606No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT250.20955574182732606No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT230.19279128248114No Hit
ACCATACACAGGCAGATGAGTATTTACATGTGTAATCACCTTCCAGATCA220.18440905280804692No Hit
CAATTATACCTAACCACAGACTGGAATTTCCTTAATAAAATGCATTGTTG220.18440905280804692No Hit
GTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAG200.16764459346186086No Hit
GTACAAATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGA200.16764459346186086No Hit
GGATAGGACAGGGTTGGTTATTGACTGAAACTGGGGATGAGGTAATGCTC180.15088013411567477No Hit
CTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACC180.15088013411567477No Hit
GTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCTAATTA180.15088013411567477No Hit
CTCTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATA170.14249790444258173No Hit
GTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGG170.14249790444258173No Hit
GTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAATTAATT170.14249790444258173No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA160.13411567476948869No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG160.13411567476948869No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT150.12573344509639564No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA150.12573344509639564No Hit
GTGTATGGTTAGGATTTGCATCTTTCACTTGCTGAATGTAAGAAGCACCA150.12573344509639564No Hit
AATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAA150.12573344509639564No Hit
GTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGAT140.1173512154233026No Hit
AATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGAACA140.1173512154233026No Hit
CTATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGCCTG130.10896898575020957No Hit
GGCAAATGCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGA130.10896898575020957No Hit
GTGCTGATTTGTACCATTATCAATTAATTTGGCCCATTCACAAACATAAC130.10896898575020957No Hit
ATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAATAA130.10896898575020957No Hit
CATATATACAGTATAGTGACTCAGCAGTTACTCAGAGCTTCTCACAGAAA120.10058675607711651No Hit
ATTCTATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGC120.10058675607711651No Hit
AATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGACTGAA120.10058675607711651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)