FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG40.30441400304414No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA40.30441400304414No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC40.30441400304414No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT40.30441400304414No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA30.228310502283105No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC30.228310502283105No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA30.228310502283105No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG30.228310502283105No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT30.228310502283105No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC20.15220700152207No Hit
GTAAAGCAAAATTATGTCCCTAAAAGATGCTGAGCTCCAGGACAATCTGA20.15220700152207No Hit
GTATGGTTAGGATTTGCATCTTTCACTTGCTGAATGTAAGAAGCACCAAT20.15220700152207No Hit
GGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGTCCATGAGGGA20.15220700152207No Hit
ATTTTCTTCTGTCTTTCCTTCCTTCTGGATTCCAGTTATACCAAATGATA20.15220700152207No Hit
GGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCG20.15220700152207No Hit
GGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGC20.15220700152207No Hit
ACCTCAGGGTCCTGGGATCAAGCCCTGTGTCACGCTCCTTGCTCGTTGGG20.15220700152207No Hit
ATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTA20.15220700152207No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20.15220700152207No Hit
TTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTC20.15220700152207No Hit
GAAACAATGTTGTCAAAACACAAACCAACCACTGAGAATTTTCAGAGTTG20.15220700152207No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC20.15220700152207No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA20.15220700152207No Hit
GGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCAGCAATCT20.15220700152207No Hit
GCCCATACAAATGGAAGGGATTATCAAGAACTTCTGGGCAGGGTTTATTC20.15220700152207No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA20.15220700152207No Hit
GTTGTTAAGTGTCTGCCTTCAGCTCAGGTCATGATTCCAAAGTCCTGGGA20.15220700152207No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT20.15220700152207No Hit
AGCTAACATTGTGTAAGCAGTGTATTTTCAGTTGTGACTCTGACTCCTTT20.15220700152207No Hit
CAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTCAGGTCATGATCCT20.15220700152207No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATCTGTCTCTTATAC20.15220700152207No Hit
GCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGAT20.15220700152207No Hit
TAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCT20.15220700152207No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA20.15220700152207No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA20.15220700152207No Hit
GTTGTTAAGTGTCTGCCTTCAGCTCAGGTCATGATCCCAGGTCCCTGTCT20.15220700152207No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.15220700152207No Hit
GTATTAGGGAACAGAACGAGTTACCAGGAAGCTCCCATACGGAATTTAGG20.15220700152207No Hit
AGAATGAAGTGGATGATGGCAATGAGATACCCAATTACCTGTCTCTTATA20.15220700152207No Hit
GCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGCTGTCTCTTATA20.15220700152207No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20.15220700152207No Hit
ATGCAAATCCTAACCATACACAGGCAGATGAGTATTTACATGTGTAATCA20.15220700152207No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC20.15220700152207No Hit
CTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGG20.15220700152207No Hit
GATTATGGCCATATGGTCCACAGTGGTCTTAGTGCTGTCTCTTATACACA20.15220700152207No Hit
ATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAAT20.15220700152207No Hit
AAACCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTG20.15220700152207No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA20.15220700152207No Hit
GAACCGTGCGAAGGCCCCTTCTCCTCAGTACCGCTGCAGCTTGGGGTTCA20.15220700152207No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20.15220700152207No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCTGTCTCTTATAC20.15220700152207No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC20.15220700152207No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC20.15220700152207No Hit
GCACGCTTCCTGCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGAC20.15220700152207No Hit
GAGGGAATTGTCTAGAAGCACTTTCAGGAGAGGCAGAGAGATGGTATCCA20.15220700152207No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTCTGTC20.15220700152207No Hit
GAAGAAATGCGGCACAGATTGAAAGCGACAGAGAATAGTTTCGAACAAAT20.15220700152207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)