FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H3_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H3_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5521
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC110.1992392682485057No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT90.1630139467487774No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG90.1630139467487774No Hit
ACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAAGGGGT80.14490128599891325No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC80.14490128599891325No Hit
CAATAATGGTCAACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACA70.1267886252490491No Hit
GTCTTAAGAATATGCTGTTAAGAGAACAAGATAAAGGGTGAGTGGGAGAG70.1267886252490491No Hit
CCTATGAACCATGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAG70.1267886252490491No Hit
TATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTAACTAACCCCT60.10867596449918493No Hit
CCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTTCGACTAA60.10867596449918493No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC60.10867596449918493No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG60.10867596449918493No Hit
GAACAATAATCACCTGATGTAAAAATCCAGTGACAACACTGACAAGCAAG60.10867596449918493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)