FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H1_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_H1_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9897
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC350.3536425179347277No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT300.3031221582297666No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG260.26270587046579774No Hit
TACATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTT230.23239365464282102No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT230.23239365464282102No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG220.22228958270182883No Hit
CCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTTCGACTAA210.2121855107608366No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA200.2020814388198444No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG200.2020814388198444No Hit
GCGCTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACC180.18187329493785995No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG180.18187329493785995No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC180.18187329493785995No Hit
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC160.16166515105587553No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC150.1515610791148833No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA150.1515610791148833No Hit
CAATTATACCTAACCACAGACTGGAATTTCCTTAATAAAATGCATTGTTG140.1414570071738911No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT140.1414570071738911No Hit
GCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGC120.12124886329190664No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA120.12124886329190664No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA120.12124886329190664No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG120.12124886329190664No Hit
CTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACC110.11114479135091442No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA110.11114479135091442No Hit
TTATCAGCCATTAGCTGGTTTCCTGTGTCCCAGCAAATGCTGAGGTAAGT110.11114479135091442No Hit
AATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGACTGAA110.11114479135091442No Hit
ACCTGAGCCAAAGGCAAATGCTTAATGATCCTCTATTGGTGCTTCTTACA110.11114479135091442No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA100.1010407194099222No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC100.1010407194099222No Hit
GTGCAATATTGGCAATCAAGACTTACCTCAGCATTTGCTGGGACACAGGA100.1010407194099222No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG100.1010407194099222No Hit
CAATAATTGGTGTCCTTTGTTAAGCGCTATTATGTGTCCTAGAAACCTAT100.1010407194099222No Hit
GTGTAATGGATGGCTGAGTTCTCAAAGGGACTGTTAGCACGGTGGAATAG100.1010407194099222No Hit
GTCTAAAAGTATTGCTCTTCGACTAACTAGAATTCCCAAGATGGGGAAGG100.1010407194099222No Hit
TGTTTGAAATGTGCAATATTGGCAATCAAGACTTACCTCAGCATTTGCTG100.1010407194099222No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA100.1010407194099222No Hit
ATTCTATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGC100.1010407194099222No Hit
AATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGAACA100.1010407194099222No Hit
GGTTAAAGGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAG100.1010407194099222No Hit
AATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAA100.1010407194099222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)