FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_G6_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_G6_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6989039
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC582390.8332905282113893No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT376610.5388580604572388No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG360400.5156645999542998No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT329480.47142389676177227No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG276350.39540486181290446No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA268850.38467377274615294No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA240760.34448226716147956No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA218120.3120886862986456No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA207390.29673607487381315No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG205350.2938172186476567No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA199850.2859477533320389No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA196010.28045343572986214No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA195880.28026743018603845No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC193820.27731995772237067No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA189230.2707525312135188No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC176620.2527099934626206No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG170980.2446402144844234No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA169090.241935980039602No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT168700.24137796340813092No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG155180.22203338685046684No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT150380.2151654898477459No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC150080.21473624628507582No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA149010.21320527757821925No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT141900.20303220514293882No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA133720.19132816400080183No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT131870.1886811620310031No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT131550.18822330223082173No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC130640.18692126342405585No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC129310.18501828362955192No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC128820.18431718581052417No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA127400.18228543294721922No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC125530.17960981473990917No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT124260.17779268365793924No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT123510.17671957475126407No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG121110.1732856262499036No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC120210.1719978955618934No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG119900.17155434388046767No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT119690.17125387338659864No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA117240.16774838429145983No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA117180.1676625355789258No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA116740.1670329783536764No Hit
GAATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG116570.16678974033483002No Hit
ATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGGACGTA114800.16425720331507665No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG113190.161953596195414No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT111700.15982168650081935No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG108940.1558726457242548No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA107810.15425582830486423No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT106280.1520666861352469No Hit
GGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTT105480.15092203663479342No Hit
GGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAA105010.150249555053277No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG104800.14994908455940795No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG104310.1492479867403802No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT103020.14740223942089892No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA102740.1470016120957402No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA99880.14290949013161897No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA99260.1420223867687675No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG99060.14173622439365413No Hit
GAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGG98490.140920661624581No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA98490.140920661624581No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG98440.14084912103080266No Hit
TCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATG96560.13815919470473695No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC96350.1378587242108679No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT95070.1360272850101423No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG94030.13453924065955275No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC93410.1336521372967013No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA93210.13336597492158792No Hit
GTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACT92180.13189223868975405No Hit
GTACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATTGGGCA92170.1318779305709984No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC89690.12832951711959256No Hit
AGGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA88510.12664115910642365No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA88200.12619760742499792No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT85420.12221995041092201No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT84540.12096083596042316No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC84240.12053159239775312No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG84190.12046005180397477No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC84100.12033127873517374No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCTGTCTC84010.12020250566637274No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA82760.11841399082191414No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG82150.11754119557781835No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG82000.11732657379648333No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA81120.11606745934598449No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACCTGTCTC80170.11470818806419594No Hit
ACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAG79450.1136780035137878No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGA76040.10879893501810477No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATTGGGCA75730.10835538333667905No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAG75340.10779736670520797No Hit
TGCAAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGT75040.10736812314253791No Hit
CCCCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT74690.10686733898608951No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT74180.10613762492955041No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA73660.10539360275425562No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC72940.1043634182038475No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA72450.10366232038481972No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT72020.10304707127832596No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT71880.1028467576157466No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT71660.10253197900312189No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT71520.10233166534054253No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA71500.1023030491030312No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC71240.10193103801538379No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT71130.10177364870907145No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG70700.10115839960257769No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC70620.10104393465253235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)