FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_G2_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_G2_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556605
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA371961.0458288170882064No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG239680.6739010938802594No Hit
GAATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG196750.5531960957148742No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC154470.4343186831261835No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG140180.3941399171400816No Hit
ATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGT131500.3697346205159133No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT104910.29497231207851304No Hit
GTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTGTCTCT104760.29455056156081433No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT103860.29202005845462176No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG98690.27748372394460447No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC98350.27652775610448727No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA88360.24843917162574986No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACCTGTCTCT86620.2435468656204442No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA85860.2414099963307705No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA84460.23747365816558208No Hit
GGGCCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT83530.23485880495584974No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCTGTTAGCCA82780.23275005236735596No Hit
CCATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCA80950.22760469605143108No Hit
ATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTG80490.22631132779715488No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG79460.2234153075756234No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA78870.22175642220600825No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT77720.21852300157031776No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT77360.21751080032784073No Hit
CTGTTAGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTCCATGGC73720.2072763210983508No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT73650.2070795041900914No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG71930.2022434315871456No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC71650.20145616395410793No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATCTG68950.19386465463553024No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG67420.18956279935500286No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA67040.188494364710166No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGCTGTCTCTTATACA66900.18810073089364718No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT66360.18658242902993163No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA66050.1857108112933542No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA64610.1816620063234461No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA64120.18028428796563015No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG63390.17823176877949617No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT62440.17556068216740403No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA62330.17525139845442494No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT61100.1717930442092951No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC60320.16959994151726154No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG59880.16836280666534517No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG59620.16763177243466731No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG57230.16091188085266708No Hit
AGATTGCTGTTAGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTC55650.15646944206624014No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGATGGCATCAATTGG55330.1555697076284828No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG55330.1555697076284828No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC55300.15548535752494302No Hit
ACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAG55090.15489490680016477No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC54060.15199888657863328No Hit
ATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAAT53490.150396234611378No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT53440.1502556511054784No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG53270.1497776671854198No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA52530.14769703129810594No Hit
GGATACACCATGATGGCATCAATTGGCTAACAGCAATCTATTCGAGAAAT52040.14631931294028996No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA51970.14612249603203054No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGATGGCATCAAT51890.1458975624225912No Hit
TCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATG51670.14527899499663302No Hit
ATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGCTGTCTCTTATA50850.14297342549987982No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT50610.1422986246715618No Hit
GTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGG50320.1414832403373442No Hit
GTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTGTCTCTTATACA49960.14047103909486716No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA49830.14010552197952822No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT49220.13839040320755328No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCCTGTCTCTTATACA48740.13704080155091725No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG48100.13524133267540253No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG47850.1345384151459046No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA47810.13442594834118493No Hit
GGGCCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTC46740.13141746131493376No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG46310.13020844316419733No Hit
ATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGGACGTA45990.12930870872643996No Hit
GTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACT45880.12899942501346087No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG45790.12874637470284162No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA45620.12826839078278301No Hit
GAATGAAGTGGATGATGGCAATGATTCCAGAGAGGAATGAACAAGGACAA45270.12728430624148593No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG44830.12604717138956953No Hit
AGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTAT43620.12264505054679954No Hit
GTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAATGTGGT43510.12233576683382044No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAG43290.12171719940786227No Hit
GTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG42990.12087369837246476No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA42980.12084558167128484No Hit
GCCATGGAACAGGAACAGGATACACCATGATGGCATCAATTGGCTAACAG42930.12070499816538525No Hit
ATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGCTGTCTCTT42830.12042383115358607No Hit
GCCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTT42690.12003019733706723No Hit
GGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTT42010.11811826165683285No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC41950.11794956144975334No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA41800.11752781093205458No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACCTGTCTCTTATACA41650.11710606041435584No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC40660.11432250699754401No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA40410.11361958946804608No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT39570.11125778656893301No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCCTG39070.10985195150993714No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA38520.1083055329450417No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC37950.1067028809777864No Hit
GTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTG37440.10526892921761061No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC36950.10389121085979468No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC36900.10375062735389506No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA36550.10276654281259796No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCTGTTAGC36100.10150129125950169No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA35710.10040473991348492No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGCTGTCTCTTATACACAT35710.10040473991348492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)