FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_F9_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_F9_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338027
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA5760.170400589302038No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA5510.16300473039135926No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC5160.15265052791640907No Hit
GTGCAGGCCCACTCATCTCCATCAGGTCATCTTTTAATTCCTCTGATGTG4920.14555050336215747No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG4500.13312546039221718No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC4310.12750460762010135No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG4210.12454626405582986No Hit
CTTTTATAACATGCACTCTTTTTTATGATAGAGGCACTGAAACTAAAGAA4190.12395459534297557No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA4160.12306709227369413No Hit
GGCCTGCACTGAGGAAGAAGGCAGAAGACGGAGAACTGTCAGGAAACAAA3680.10886704316519094No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT3490.10324619039307512No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT3420.10117534989808506No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)