FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_F9_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_F9_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296431
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA5060.17069739669602707No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA4740.1599023044148554No Hit
CTTTTATAACATGCACTCTTTTTTATGATAGAGGCACTGAAACTAAAGAA3690.12448090786726085No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT3580.12077009489560807No Hit
GTGCAGGCCCACTCATCTCCATCAGGTCATCTTTTAATTCCTCTGATGTG3440.11604724202259548No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG3140.10592684300899703No Hit
CATATATACAGTATAGTGACTCAGCAGTTACTCAGAGCTTCTCACAGAAA3130.10558949637521041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)