FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_F8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_F8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACAAAAGTATACACAGAAGACACAGACTGGAAATAAATACTTGAC27.4074074074074066No Hit
GTCCTGAGGTTTAATGGGGCGCCCACAGCCAGTTTCCAACAAGACGTGGG27.4074074074074066No Hit
GAAGATACACAACAGGTTGCTGGAAACATAGGAAACCAGAAAATGGGTAT13.7037037037037033No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT13.7037037037037033No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT13.7037037037037033No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG13.7037037037037033No Hit
ATCTCACCTGCTTCTTTTTATGGTGCTTTTCTCTCCTGACTGCTTATTAA13.7037037037037033No Hit
CCCCAAGGCAGGTCCAGGTGGACCAGGGATTGCTCACATTCTGCTGCAGC13.7037037037037033No Hit
GGGGGAGTGACAGTCACAGTCAGACTCACAACCACGATCACCCCTGACCA13.7037037037037033No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG13.7037037037037033No Hit
GTATAGTGACTCAGCAGTTACTCAGAGCTTCTCACAGAAAGTGTACTCCT13.7037037037037033No Hit
CTTCTGGCTAAGCAGGGAGCCCGATAGGGGGCTCAATCCCAGGACGCTGG13.7037037037037033No Hit
AGACAAGAGTATGGTGAGGGATGGCCGGTAAGCCATCCCTCATAAATCCC13.7037037037037033No Hit
TTCTGTAGCTATTAAGAAAGAATGTTGAAGCTGTGCATGTCCTATCCTGG13.7037037037037033No Hit
AGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGTGAAATTAAACTC13.7037037037037033No Hit
GTTTTGTTAAGGTGGCACAGGAGGTCCTTTAAACCTAATCCTCGCTGGGC13.7037037037037033No Hit
TGACATATCAAAACTTATAAGCTGTATATAAAGCTGTCTAGAGGAAATTG13.7037037037037033No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA13.7037037037037033No Hit
ATTAAAAGATGACCTGATGGAGATGAGTGGGCCTGCACTGAGGAAGAAGG13.7037037037037033No Hit
GTCTTAAGTGTCTGCCTTCAGCTCAGGTCATGATCCCAAGGTCTGTCTCT13.7037037037037033No Hit
GATCGAGCCCCGCATCCGGCTCCCTGCTCAGCGGAAGCCTGCTTCCCCCT13.7037037037037033No Hit
GGCTCGATCCCAGGACCCTGGGATCATGACCTGAGCTGAAGGCAGATGCT13.7037037037037033No Hit
GTAGAGGTAGAGGGAGAAGCAGGCTCCCTGCTGAGCCTGTCTCTTATACA13.7037037037037033No Hit
AGTAGAAACAAGGTGGTGTTCGTAAGCCCTGGTGTGTGTTCAGGAAGTAG13.7037037037037033No Hit
GGCTGGGACCATGGTTTTAGGATGGGGAGACCCAGACTCTGGGTGTCAGA13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)