FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_F2_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_F2_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544444
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA370861.0463136108230233No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG238870.6739279841915967No Hit
GAATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG195950.5528370599168727No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC154030.4345674526103389No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG139650.3939969146077636No Hit
ATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGT131020.369648949172282No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT104650.29525082072110603No Hit
GTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTGTCTCT104370.2944608519700128No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT103460.2918934535289597No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG98380.27756116333055336No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC98040.2766019155613687No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA88180.24878373025501319No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACCTGTCTCT86210.24322573582767848No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA85620.24156115881644627No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA84120.23732918336416092No Hit
GGGCCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT83220.23478999809278972No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCTGTTAGCCA82540.23287150255442038No Hit
CCATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCA80510.22714422910899423No Hit
ATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTG80020.22576178379458103No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG79200.22344830388066508No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA78620.22181194003911475No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT77410.21839814650760456No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT77060.21741068556873802No Hit
CTGTTAGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTCCATGGC73390.20705645229547992No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT73300.2068025337683428No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG71660.20217557394051083No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC71360.20132917885005377No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATCTG68750.1939655415630773No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG67160.18947964758365488No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA66870.18866146566287972No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGCTGTCTCTTATACA66650.18804077592987786No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT66130.18657369110641894No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA65850.1857837223553257No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA64370.18160817324240416No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA63930.18036679377640047No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG63100.17802510069280258No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT62350.17590911296665993No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA62090.17517557055493047No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT60810.171564284835647No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC60200.16984328148505096No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG59660.16831977032222825No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG59390.16755801474081689No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG57050.1609561330352518No Hit
AGATTGCTGTTAGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTC55540.15669594441328458No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC55200.1557366966440999No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG55180.15568027030473608No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGATGGCATCAATTGG55110.15548277811696276No Hit
ACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAG54920.15494672789300662No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC53880.1520125582460888No Hit
ATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAAT53350.150517260252948No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT53300.15037619440453848No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG53030.1496144388231271No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA52440.1479498618118949No Hit
GGATACACCATGATGGCATCAATTGGCTAACAGCAATCTATTCGAGAAAT51830.14622885846129885No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGATGGCATCAAT51760.14603136627352556No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA51740.14597493993416175No Hit
TCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATG51530.1453824633708418No Hit
ATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGCTGTCTCTTATA50610.1427868517601068No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT50450.14233544104519635No Hit
GTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGG50160.1415172591244212No Hit
GTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGCTGTCTCTTATACA49830.14058622452491842No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA49680.1401630269796899No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT49110.13855487630782148No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCCTGTCTCTTATACA48530.13691851246627115No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG47950.13528214862472082No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA47690.13454860621299136No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG47620.13435111402521807No Hit
GGGCCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTC46610.13150158388734595No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG46140.13017556491229654No Hit
ATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGGACGTA45790.12918810397342995No Hit
GTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACT45760.12910346446438425No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG45600.1286520537494738No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA45450.1282288562042453No Hit
GAATGAAGTGGATGATGGCAATGATTCCAGAGAGGAATGAACAAGGACAA45040.12707211624728731No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG44700.12611286847810263No Hit
AGCCAATTGATGCCATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTAT43460.12261443543754676No Hit
GTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGGGAATGTGGT43400.12244515641945536No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAG43120.12165518766836209No Hit
GTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG42830.12083700574758692No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA42830.12083700574758692No Hit
GCCATGGAACAGGAACAGGATACACCATGATGGCATCAATTGGCTAACAG42760.12063951355981362No Hit
ATCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGCTGTCTCTT42740.1205830872204498No Hit
GCCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTT42520.11996239748744797No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC41850.11807211511876052No Hit
GGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTT41830.11801568877939672No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA41740.1177617702522596No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACCTGTCTCTTATACA41510.11711286734957585No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC40570.11446082939947705No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA40320.1137555001574295No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT39420.11121631488605829No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCCTG38920.10980565640196319No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA38350.10819750573009476No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC37780.10658935505822634No Hit
GTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTG37360.10540440193158646No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC36820.10388089076876372No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC36820.10388089076876372No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA36460.10286521666021525No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCTGTTAGC36010.10159562402452967No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGCTGTCTCTTATACACAT35640.10055173674629928No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA35630.10052352357661737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)