FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E8_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E8_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGGGAGGGGAGGAGAAAGAAAACAAATCTTGAAATTTAGTCTTCT13.0303030303030303No Hit
AGCGTCTGCCTTCGGCTCACGTCATGATCCCAGGGTCCTGGGACCGAGGC13.0303030303030303No Hit
CTTAAACAAATTAATCCAAATATCACAATAAAATTGACTAACAACCACAT13.0303030303030303No Hit
ATACCAAATCATTACGGTAGACACCAGAACTCATAGAAGGTTAATGTCAA13.0303030303030303No Hit
CCCATTTAGGGCCTTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCAC13.0303030303030303No Hit
AACCACAACAACAAAAAAAAAAAAATTAAAAAAAACAAACACAAAACAAG13.0303030303030303No Hit
ATTCCAAATATTCAACATTCTTCATAGTGACTAAATCCAAATTAATCAAA13.0303030303030303No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA13.0303030303030303No Hit
AGCGAAAGCAGGACCCCAGGACCATGACCTGAGCCGAAGGCAGCTGCCCA13.0303030303030303No Hit
GAGTGGGAGAGAGAGAAGCAAGCTTCCTGCTGAGCAAGAACCCGATGTGG13.0303030303030303No Hit
CTCTAGGTCATGGTCTCTTGAGGAGGTGGAACCACTGGCTGGGATGGTGG13.0303030303030303No Hit
ACAGTGCTCCGCAGCCCCTACCGCTGCCCGCCTCGGTTACTTTGCTGACC13.0303030303030303No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCAATACCAACCTGTCTC13.0303030303030303No Hit
CGGTGAGCAGGGAGCCCGATGCCGGGCTCGATCCCAGGACCCAAGGAGCT13.0303030303030303No Hit
GTGTGGACGTGGGTGTGTGCATTTTAAAATATTATATATAGAGAAAGATT13.0303030303030303No Hit
ATAATACTACCTTTACTATTCACAAACTCATAACAAAAAAAAACACTAAA13.0303030303030303No Hit
CATTAAACTCAGAAACGCAGAAACGGCTTAGAGCCCTGTCAGCGTCCCTG13.0303030303030303No Hit
ACATGGAAAATTCGATAATTTGTCTATAAAATAACCAAAAGAATAGACAC13.0303030303030303No Hit
CTATAGTGTACTCAATAAATGTGAACTGTTTTATTGGCAAATGAGTAAAC13.0303030303030303No Hit
TGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTTAATTTCACATGACTG13.0303030303030303No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA13.0303030303030303No Hit
AGGTATTAGAAAGCCATTGGCGGCCTGTGGTGTGACATGGTAGGCTATTA13.0303030303030303No Hit
GCTACTTTAAAAGGTGCATTAGATTTATCCCTACCCAGGACCTATTATTT13.0303030303030303No Hit
GACCCAAGCCGAAGGCAGACGCTTAATGACTGAGCCACCCAGGCGTCCCA13.0303030303030303No Hit
GTTGGTTCTATTGGAAAATGAAAGAACATAGGACTACCACGATTCAAATG13.0303030303030303No Hit
GCACTGGGGTCTTCAGAAAACCCTTTCAACAGAAGGAAATGGCACGATTC13.0303030303030303No Hit
AAAATAGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCCTG13.0303030303030303No Hit
CTTGGGAATCCAGGAATGTGTAGTTGCAAAGGCATAATCTTCTCTTTACT13.0303030303030303No Hit
GCAAAAGCAGGTACCATTATATTTCCAGGAACAGGAAGCATATCAGTGTT13.0303030303030303No Hit
GGTCAATTATATTCAATATGGAGAGAAAAAAAGAATTCGGATGGTCATCA13.0303030303030303No Hit
GGTCATGATCTCAAGGTCCTGGGATCAAGTCCCCAATCAGGTTCCTTGCC13.0303030303030303No Hit
CACATACAGATGGCTACTACCACCAATCCACTAATCAGGCATGAAAACAG13.0303030303030303No Hit
CAAATATATTTTATATTTAATCAAATTAATAAAAAAACACCATAAACAAT13.0303030303030303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)