FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC120.5427408412483039No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG80.3618272274988693No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC60.27137042062415195No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT50.22614201718679333No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA50.22614201718679333No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA40.18091361374943465No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC40.18091361374943465No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT40.18091361374943465No Hit
AGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTA40.18091361374943465No Hit
CGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCT40.18091361374943465No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG40.18091361374943465No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT30.13568521031207598No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT30.13568521031207598No Hit
GAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAA30.13568521031207598No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA30.13568521031207598No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA30.13568521031207598No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA30.13568521031207598No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA30.13568521031207598No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA30.13568521031207598No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC30.13568521031207598No Hit
GGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTC30.13568521031207598No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG30.13568521031207598No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCCTGATGTGTA30.13568521031207598No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.13568521031207598No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC30.13568521031207598No Hit
GCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTT30.13568521031207598No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.13568521031207598No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.13568521031207598No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA30.13568521031207598No Hit
AGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCAT30.13568521031207598No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA30.13568521031207598No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA30.13568521031207598No Hit
ATTGTCTGCAAATCGCTTGCGGATATGCCAAAGGAAACAGTCTACCTGAA30.13568521031207598No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT30.13568521031207598No Hit
GAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC30.13568521031207598No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT30.13568521031207598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)