FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC40.2526847757422615No Hit
AGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGCTGTCTCTTATACA30.18951358180669614No Hit
GAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCAT30.18951358180669614No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT30.18951358180669614No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC20.12634238787113075No Hit
CCACATGGGGCTCCTTGCTCACCAGGGAGCCTGCTTCCCCCTAGGCCTGA20.12634238787113075No Hit
GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGCGATAACACGT20.12634238787113075No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCCTGTCTCTTAT20.12634238787113075No Hit
CCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCG20.12634238787113075No Hit
GATCTTGAAAGAGGAATCCAGCGAGACACTTAGAATGACAATTGCATCTG20.12634238787113075No Hit
GGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCCCATTC20.12634238787113075No Hit
GCCTTCCACTTATTCAAGACCGCCTTTGACTTCTTTTATAGCATGCTGTC20.12634238787113075No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG20.12634238787113075No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT20.12634238787113075No Hit
AAAGAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAA20.12634238787113075No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA20.12634238787113075No Hit
GCTCAGTCAGTAAGCGTCTGCCTTCAGCTCAGGTCATGATCCCAGGGTCC20.12634238787113075No Hit
GTATAGTGACTCAGCAGTTACTCAGAGCTTCTCACAGAAAGTGTACTCCT20.12634238787113075No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA20.12634238787113075No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA20.12634238787113075No Hit
GAAGGAGTGCCTGAGTCCATGAGGGAAGAATATCAACAGGAACAGCAGAG20.12634238787113075No Hit
TACCAGAAGCGAATGGGAGTGCAGATGCAGCGATTCAAGTGATCCTCTCG20.12634238787113075No Hit
GCAAATTTCAAAGGACTGAAATCACGCACAGAGACTGTTACGGCTGTCTC20.12634238787113075No Hit
CAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTG20.12634238787113075No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA20.12634238787113075No Hit
GAAAGTGTACTCCTAATCCCCTGCTTTTGCTCCTGTCTCTTATACACATC20.12634238787113075No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT20.12634238787113075No Hit
CTACTGTAGAGGATCTAAATGTAGCCTAGCATCCAGGGTTTATCACCTGT20.12634238787113075No Hit
AGCCTGGAGTGTAAGGAGGGTTGGGGTTTGGAAAGAGAGGAGGTGAGAGA20.12634238787113075No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT20.12634238787113075No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG20.12634238787113075No Hit
CATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCAGAGG20.12634238787113075No Hit
GCAAAAGCAGGGGGAAGAAGCAGAGGGAGAAGCAAGCTTCCCACTGAGCA20.12634238787113075No Hit
GTTGGGGTCCTCATCGGAGGACTTGAATGGAATGGTAACACGGTTCGAGT20.12634238787113075No Hit
GAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGG20.12634238787113075No Hit
TCTCACAGAAAGTGTACTCCTAATCCCCTGCTTTCGCTCCTGTCTCTTAT20.12634238787113075No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA20.12634238787113075No Hit
GCTTAATGCTGGGCCATATCTCTTGCCTTCCTGATGTGTACTTTTTGATT20.12634238787113075No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT20.12634238787113075No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG20.12634238787113075No Hit
AAGCAGGGGAGAAGGGTAGAGGTAGAGGGAGAAGCAGGCTCCCTGCTGAG20.12634238787113075No Hit
GTCTCACAGACAGATGGCTACTACCACCAATCCACTAATCAGGCATGAAA20.12634238787113075No Hit
TCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTAT20.12634238787113075No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.12634238787113075No Hit
ATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTG20.12634238787113075No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT20.12634238787113075No Hit
GATCAAGTCCCCAATCAGGTTCCTTGCCTAGCAGGGAGTCTGCTTTCTGT20.12634238787113075No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT20.12634238787113075No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC20.12634238787113075No Hit
GCCTGTGTATGGTTAGGATTTGCATCTTTCACTTGCTGAATGTAAGAAGC20.12634238787113075No Hit
GTAAAGACAAGTGGGTAGCGTATAACACAGCTTATCAGAGTTTTTTCTCT20.12634238787113075No Hit
GGCTTCCTGTAGAGCAGGGAGCCCAGTGCGGAGCTCCGCCCCAGATCCCT20.12634238787113075No Hit
GTACTGTGTTCCCATTCGGTACCCCGAAGAACCCCGAAGAACCCCGCGGT20.12634238787113075No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT20.12634238787113075No Hit
ATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTT20.12634238787113075No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCTG20.12634238787113075No Hit
GAAGGCAGATGCTTAACGGAGTAAGCCACCCAGGTGTCACAGCATGGCCC20.12634238787113075No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT20.12634238787113075No Hit
GTGGATGGATGCCCCAGAAAACTGGCCCAAACATCTGCCAACACAGTCTC20.12634238787113075No Hit

[FAIL]Adapter Content

Adapter graph