FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E4_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E4_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7613450
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA316290.41543584051908133No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG264100.34688610288371236No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC258590.3396489108091601No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG223980.2941898876330704No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC209610.275315395779837No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA191710.2518043725249394No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT190690.25046463823890613No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG183420.24091574778845334No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG170440.22386697226618682No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT167570.22009732775548535No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC139520.18325463488957042No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA133550.17541324892131688No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT133030.17473024712843718No Hit
TCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATG122240.16055795992618327No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG117790.15471304073711656No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA116690.1532682292521787No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT115330.151481916870801No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG113950.14966933518969716No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC106550.13994969429102444No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT105750.13889892230197873No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT104500.13725709106909484No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA102530.13466956504606978No Hit
GGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTT101480.1332904268104473No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA100820.1324235399194846No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG100180.13158292232824803No Hit
GTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACT99020.13005930294413176No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG98680.12961272484878736No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG98490.129363166501389No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA97940.1286407607589201No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA96850.1272090839238453No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG96340.12653921678082866No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC94400.12399109470739283No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC94150.12366272846081605No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA93540.12286151481916872No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT93180.12238866742409814No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG92920.12204716652765829No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT92220.1211277410372433No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC91920.12073370154135116No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT89810.11796229042024312No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA88690.11649120963557913No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA86860.11408756871063709No Hit
GTTCAACTCCTTCCTCACACATGCACTGAAGTAGTTGTGGCAATGCTACT86150.11315500857035904No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG83940.11025225095062027No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA81170.10661395293854953No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG79150.10396075366620915No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA76270.10017797450564461No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)